Human/Mouse/Rat Cytochrome c Alexa Fluor™ Plus 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB899AFP488

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FAB899AFP488-100UG
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 2.7D5

View all Cytochrome c Antibodies »

Product Specifications

Immunogen

Pigeon Cytochrome c

Specificity

Detects native human, mouse, and rat Cytochrome c.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications

Application
Recommended Usage

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Cytochrome c

Cytochrome c is a critical mitochondrial outer membrane-associated protein in the electron transport chain (1).  Reduction and oxidation of an iron molecule (Fe3+ to Fe2+ and back) within its central heme group allow it to receive an electron from the cytochrome c1 subunit of cytochrome reductase and pass it to cytochrome a within the cytochrome oxidase complex (1).  Release of cytochrome c from mitochondria occurs during initiation of apoptotic pathways, either by mechanisms that rupture or that do not rupture the mitochondial membrane (2, 3).  Once in the cytosol, cytochrome c forms a complex with procaspase 9, recruiting Apaf-1 (apoptotic protease activating factor-1) and dATP to form apoptosomes, which activate caspase and ultimately destroy the cell (2, 3).  Cytochrome c is highly conserved, with 90% or greater amino acid identity among human, mouse, rat, cow and dog sequences (4).  After cytochrome c translation, the initial methionine is cleaved to form the 104 amino acid mature form.

References

  1. Zubay, G. L., 1983, “Biochemistry”, pp. 380, Addison-Wesley Pub. Co, Inc.
  2. Liu, X. et al., 1996, Cell 86:147.
  3. Li, P. et al., 1997, Cell 91:479.
  4. Matsubara, H and E. L. Smith, 1963, J. Biol. Chem. 238:2732.

Alternate Names

CYCS

Entrez Gene IDs

54205 (Human)

Gene Symbol

CYCS

Additional Cytochrome c Products

Product Documents

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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