Human/Mouse/Rat BAT3/BAG6 Alexa Fluor® 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6438R
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Western Blot
Label
Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human BAT3/BAG6
Met1-Gln190
Accession # P46379
Met1-Gln190
Accession # P46379
Specificity
Detects human, mouse, and rat BAT3/BAG6 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: BAT3/BAG6
BAT3 (HLA-B-associated transcript 3; also BAG6, BAG family molecular chaperone regulator 6, Protein G3 and Scythe) is a multifunctional molecule that is not easily placed into known protein families. Although its predicted MW is 119 kDa, it runs anomalously in SDS-PAGE at 150-170 kDa. BAT3 is found in diverse cell types such as dendritic cells, hepatocytes, renal epithelial cells and tumor cells. It is found extracellularly where it is complexed to Hsp70, and activates the NKp30 receptor expressed on NK cells. Intracellularly, it binds to TGF-beta receptors I and II where, upon receptor ligation, it translocates to the nucleus and activates TGF-beta target genes. Human BAT3 is 1132 amino acids (aa) in length. It contains one Ub-like domain (aa 17-77), two Pro-rich regions (aa 195-273 and 394-681), an NLS (aa 1030-1052) plus a BAG domain (aa 1053-1101). BAT3 is phosphorylated on multiple Ser/Thr residues. Caspase 3 will cleave BAT3 after Asp1001, generating a 20 kDa C-terminal fragment. There are also multiple splice variants. Two of them share an absence of aa 185-190 that may be accompanied by a 36 aa insertion after Gly489. Over aa 1-190, human BAT3 shares 93% aa identity with mouse BAT3.
Long Name
Large proline-rich protein BAT3
Alternate Names
BAG6, D6S52E, Protein G3, Scythe
Gene Symbol
BAG6
UniProt
Additional BAT3/BAG6 Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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