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Human/Mouse Preso/FRMPD4 Antibody

Catalog # MAB7676 | R&D Systems, Inc. a Bio-Techne Brand
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MAB7676
MAB7676-SP

Key Product Details

Species Reactivity

Human, Mouse

Applications

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 809408

Product Summary for Human/Mouse Preso/FRMPD4 Antibody

Immunogen

E. coli-derived recombinant human Preso/FRMPD4
Asn890-Ser1022
Accession # Q14CM0

Specificity

Detects human Preso/FRMPD4 in direct ELISAs and human and mouse Preso/FRMPD4 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse Preso/FRMPD4 Antibody

Detection of Human and Mouse Preso/FRMPD4 antibody by Western Blot.

Detection of Human and Mouse Preso/FRMPD4 by Western Blot.

Western blot shows lysates of IMR-32 human neuroblastoma cell line and bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Preso/FRMPD4 Monoclonal Antibody (Catalog # MAB7676) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Preso/FRMPD4 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Preso/FRMPD4 antibody by Simple WesternTM.

Detection of Mouse Preso/FRMPD4 by Simple WesternTM.

Simple Western lane view shows lysates of bEnd.3 mouse endothelioma cell line, loaded at 0.5 mg/mL. A specific band was detected for Preso/FRMPD4 at approximately 170 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse Preso/FRMPD4 Monoclonal Antibody (Catalog # MAB7676). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse Preso/FRMPD4 Antibody

Application
Recommended Usage

Simple Western

20 µg/mL
Sample: bEnd.3 mouse endothelioma cell line

Western Blot

2 µg/mL
Sample: IMR‑32 human neuroblastoma cell line and bEnd.3 mouse endothelioma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 1 publication using MAB7676 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Preso/FRMPD4

Preso, also known as FRMPD4 and PDZD10, is a scaffolding protein that is widely expressed in the brain as 140 kDa and 170 kDa species. It contains one WW domain (aa 33-66) one PDZ domain (aa 78-155), and one FERM domain (aa 204-519). The 140 kDa species is enriched in post-synaptic densities where it interacts with PSD-95, SAP97, SAP102, and F-actin. Preso promotes dendritic spine extension and maintenance as well as excitatory synaptic transmission. Within aa 890-1022, human Preso shares approximately 94% aa sequence identity with mouse and rat Preso.

Long Name

FERM and PDZ Domain Containing 4

Alternate Names

FRMPD4, PDZD10, PDZK10

Entrez Gene IDs

9758 (Human); 333605 (Mouse); 302656 (Rat)

Gene Symbol

FRMPD4

UniProt

Product Documents for Human/Mouse Preso/FRMPD4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse Preso/FRMPD4 Antibody

For research use only

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Reconstitution Calculator

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