Human/Mouse Oct-3/4 Alexa Fluor™ Plus 680-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB1759AFP680
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready
Label
Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)
Antibody Source
Monoclonal Rat IgG2B Clone # 240408
Product Specifications
Immunogen
E. coli-derived recombinant human Oct‑3/4
Specificity
Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Applications
Application
Recommended Usage
CyTOF-ready
Optimal dilution of this antibody should be experimentally determined.
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
Intracellular Staining by Flow Cytometry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Oct-3/4
Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells.(2, 3) Oct-3/4 is required to sustain stem cell self-renewal and pluripotency.(4) It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.
References
- Takeda, J. et al. (1992) Nucleic Acids Res. 20:4613.
- Scholer, H.R. et al. (1989) EMBO J. 8:2543.
- Rosner, M.H. et al. (1990) Nature 345:686.
- Niwa, H. et al. (2000) Nat. Genet. 24:372.
Long Name
Octamer-binding Protein 3/4
Alternate Names
3-Oct, Oct4, Otf3g, Otf4, Pou5f1
Gene Symbol
POU5F1
UniProt
Additional Oct-3/4 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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