Human/Mouse NPM1 Alexa Fluor™ Plus 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5205AFP647
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human NPM1
Specificity
Detects human and mouse NPM1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: NPM1
NPM1 (Nucleophosmin 1; also nucleolar protein 23) is a 40 kDa member of the nucleoplasmin family of proteins. It is found in the nucleus where it performs multiple functions. NPM1 normally associates with unduplicated centrosomes. After phosphorylation at Thr199, it dissociates from centrosomes, allowing for centrosome duplication. NPM1 also negatively regulates GCN5-dependent histone acetylation during mitosis. Human NPM1 is 294 amino acids (aa) in length. It contains a Met-rich region (aa 1‑9), two acidic regions (aa 120‑132 and 161‑188) and two NLSs (aa 152‑157 and 191‑197). Phosphorylation occurs at Ser4/10/70/254. Splice variants show a deletion of aa 195‑223, a two aa substitution for aa 258‑294 and an 11 aa substitution for aa 288‑294. Over aa 9‑158, human NPM1 is 97% aa identical to mouse NPM1.
Long Name
Nucleophosmin/Nucleolar Phosphoprotein B23
Alternate Names
B23, Nucleophosmin, Numatrin
Gene Symbol
NPM1
UniProt
Additional NPM1 Products
Product Specific Notices
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
Recommended applications are based on validated applications from the unconjugated base product AF5205.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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