Human/Mouse Nodal Antibody

Name: Human/Mouse Nodal Antibody
Brand: R&D Systems
SKU: AF1315
Rating: 3 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1315

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Species Reactivity:	Human, Mouse
Immunogen:	E. coli-derived recombinant mouse Nodal His245-Leu354 Accession # P43021
Applications:	Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human and mouse Nodal in direct ELISAs and Western blots.

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Applications for Human/Mouse Nodal Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human Nodal (Catalog # 3218-ND) Recombinant Mouse Nodal (Catalog # 1315-ND)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Nodal

Nodal is a secreted protein that is a member of the Transforming Growth Factor-beta (TGF-beta ) superfamily. Nodal was named for its localized expression in the mouse node during gastrulation, and is first detected in the early primitive streak during mesoderm formation. Expression of the Nodal gene occurs asymmetrically in the left, but not right, lateral plate during somitogenesis. Nodal proteins play crucial roles in mesoderm formation and both anterior-posterior and left-right axis formation during vertebrate development. Members of the Nodal gene family include mouse Nodal, chick cNR-1, frog Xnr1-4, and zebrafish cyclops. Biologically active Nodal is a disulfide-linked homodimer and contains all seven of the cysteine residues necessary for formation of the “cysteine knot” characteristic of TGF-beta -related molecules. Mouse Nodal is 34 - 39% homologous in the conserved region to other TGF-beta superfamily members. Nodal has been shown to signal through a mechanism related to the Activin pathway, and signaling is mediated through both Smad2 and 3. Nodal signaling utilizes type II Activin receptors, together with ALK4/ActRIB, or the orphan type I receptor ALK7. Nodal interacts extracellularly with members of other protein families, including Cerberus, Lefty, and EGF-CFC ligands, such as Cripto. While the Cerberus and Lefty families act as Nodal antagonists, the EGF-CFC molecules act as co-receptors to facilitate Nodal signaling. The resulting concert of regulated Nodal activity allows for the precise control of mesoderm formation, neural patterning, and axis positioning and patterning during early vertebrate development.

Item	Value
References	Kumar, A. et al. (2001) J. Biol. Chem. 276:656. Reissmann, E. et al. (2001) Genes & Dev. 15: 2010. Schier, A. and M. Shen (1999) Nature 403:385. Zhou, X. et al. (1993) Nature 361:543.
Entrez Gene IDs	4838 (Human); 18119 (Mouse)
Alternate Names	BMP-16, MGC138230, Nodal, nodal homolog, nodal homolog (mouse), nodal, mouse, homolog

Citations for Human/Mouse Nodal Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse Nodal Antibody include:

Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Papageorgiou I et al. (2009), Expression of nodal signalling components in cycling human endometrium and in endometrial cancer. Reprod. Biol. Endocrinol., 7(0):122.
PMID: 19874624

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Human/Mouse Nodal Antibody

Name: Anonymous

Sample Tested: purified R&D Nodal protein sample

Species: Bacterial

Verified Customer | Posted 11/30/2015

This Nodal ab is much better than the other Nodal antibodies we have used. To see the quality of it, we run a western with R&D Systems purified Nodal protein (made in E.Coli). It is definitely not the best antibody, but not the worst either...

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Human/Mouse Nodal Antibody

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Formulation, Preparation and Storage

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Background: Nodal

Citations for Human/Mouse Nodal Antibody (1)

Customer Reviews for Human/Mouse Nodal Antibody (1)

Human/Mouse Nodal Antibody

Product Documents for Human/Mouse Nodal Antibody

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