Nbs1 was immunoprecipitated from 500 µg of K562 human chronic myelogenous leukemia cell line lysate following incubation with 2 µg Mouse Anti-Human/Mouse Nbs1 Mono-clonal Antibody (Catalog # MAB15731) for 1 hour on ice. Nbs1-antibody complexes were absorbed using goat anti-mouse IgG-agarose (Sigma). Immunoprecipitated Nbs1 was detected by Western blot using 1 µg/mL Human/Mouse/Rat Nbs1 Mono-clonal Antibody (Catalog # MAB1573). View our recommended buffer recipes for immunoprecipitation.

Applications for Human/Mouse Nbs1 Antibody

Application

Recommended Usage

Immunoprecipitation

2 µg/500 µg cell lysate
Sample: K562 human chronic myelogenous leukemia cell line, see our available Western blot detection antibodies

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nbs1

The Nijmegen Breakage Syndrome 1 (Nbs1) protein, also called Nibrin, is a member of the Mre11/Rad50/Nbs1 (MRN) protein complex that binds to DNA double-strand breaks in cells exposed to DNA damaging agents. In addition, the MRN complex colocalizes with replication forks during DNA replication. The MRN complex plays an important role in routine cell cycle progression and genotoxic stress responses by facilitating DNA repair. In fact, mutation of the nbs1 gene and resultant loss of Nbs1 protein expression in humans results in the chromosomal instability disease, Nijmegen Breakage Syndrome.

Long Name

Nijmegen Breakage Syndrome 1

Alternate Names

NBN, Nibrin, p95

Entrez Gene IDs

4683 (Human); 27354 (Mouse); 85482 (Rat)

Gene Symbol

NBN

UniProt

O60934

Additional Nbs1 Products

Product Documents for Human/Mouse Nbs1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse Nbs1 Antibody

For research use only

Citations
Reviews

Human/Mouse Nbs1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB15731

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Nbs1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse Nbs1 Antibody

Immunoprecipitation of Human Nbs1.