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Human/Mouse IL-5 Antibody

Bio-Techne includes R&D Systems | Catalog # MAB405

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Guinea Pig

Applications

CyTOF-reported, ELISA Capture (Matched Antibody Pair), Intracellular Staining by Flow Cytometry, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # TRFK5

Product Summary for Human/Mouse IL-5 Antibody

Immunogen

Mouse T cell clone-derived partially purified recombinant mouse IL-5

Specificity

Detects human and mouse IL-5 in ELISAs. In sandwich immunoassays with BAM6051 as the detection antibody, no cross-reactivity with recombinant human (rh) IL-3, rhGM-CSF, or recombinant mouse (rm) IL-5 is observed. In sandwich ELISAs with BAM705 as the detection antibody, no cross-reactivity with rhIL-5, rmIL-3, or rmGM-CSF is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Human/Mouse IL-5 Antibody

Detection of IL-5 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of IL‑5 in Human PBMCs by Flow Cytometry.

Human peripheral blood monocytes treated with 50 ng/mL PMA and 500 ng/mL Calcium Ionomycin for 6 hours were stained with Rat Anti-Human/Mouse IL-5 Monoclonal Antibody (Catalog # MAB405) followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B) and Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # MAB005). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Cell Proliferation Induced by IL‑5 and Neutralization by Human/Mouse IL‑5 Antibody.

Cell Proliferation Induced by IL‑5 and Neutralization by Human/Mouse IL‑5 Antibody.

Recombinant Mouse IL-5 (Catalog # 405-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human/Mouse IL-5 Monoclonal Antibody (Catalog # MAB405). The ND50 is typically 0.004-0.015 µg/mL.

Applications for Human/Mouse IL-5 Antibody

Application
Recommended Usage

CyTOF-reported

This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood monocytes treated with PMA and Calcium Ionomycin, fixed with paraformaldehyde and permeabilized with saponin

Neutralization

Measured by its ability to neutralize IL‑5-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.004-0.015 µg/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑5.

Human IL-5 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse IL-5 Biotinylated Antibody (Catalog # BAM705)
  • Standard: Recombinant Mouse IL-5 Protein (Catalog # 405-ML)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using MAB405 in the following applications:

Published Applications

Read 15 publications using MAB405 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-5

IL-5 is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentation, and activation of eosinophils. It binds to a receptor complex consisting of one IL-5 specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3.

Long Name

Interleukin 5

Alternate Names

BCDF mu, BCGFII, EDF, Eo-CSF, IL5, TRF

Entrez Gene IDs

3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)

Gene Symbol

IL5

Additional IL-5 Products

Product Documents for Human/Mouse IL-5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse IL-5 Antibody

For research use only

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