Human/Mouse IL-1ra/IL-1F3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB480
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA Capture
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 40007
Product Specifications
Immunogen
E. coli-derived recombinant mouse IL-1ra
Arg27-Gln178
Accession # P25085
Arg27-Gln178
Accession # P25085
Specificity
Detects human and mouse IL-1ra in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Human/Mouse IL-1ra/IL-1F3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human IL-1ra/IL-1F3 (Catalog # 280-RA)
Recombinant Mouse IL-1ra/IL-1F3 (Catalog # 480-RM)
Sample: Recombinant Human IL-1ra/IL-1F3 (Catalog # 280-RA)
Recombinant Mouse IL-1ra/IL-1F3 (Catalog # 480-RM)
Reviewed Applications
Read 2 reviews rated 4.5 using MAB480 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1ra/IL-1F3
IL-1ra (IL-1 receptor antagonist) is a widely expressed acute phase protein that serves to dampen inflammation. IL-1ra binds to both IL-1 RI and IL-1 RII. It competitively inhibits IL-1 (alpha or beta) binding to IL-1 RI and does not trigger recruitment of the accessory molecule IL-1 R AcP or activation of IL-1 RI signaling. IL-1ra blocks the proinflammatory actions of IL-1 including Prostaglandin E2 and IL-6 release from endothelial cells, fever generation, thrombocytosis, the production of hepatic acute phase proteins, and neutrophil expansion and infiltration.
Long Name
Interleukin 1 Receptor Antagonist
Alternate Names
DIRA, ICIL-1ra, IL-1F3, IL-1ra3, IL-1RN, IL1ra, IL1RN, MVCD4
Gene Symbol
IL1RN
UniProt
Additional IL-1ra/IL-1F3 Products
Product Documents for Human/Mouse IL-1ra/IL-1F3 Antibody
Product Specific Notices for Human/Mouse IL-1ra/IL-1F3 Antibody
For research use only
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