Human/Mouse HSP27 Antibody

Name: Human/Mouse HSP27 Antibody
Brand: R&D Systems
SKU: AF1580
Price: 186 USD

Catalog # AF1580 | R&D Systems, Inc. a Bio-Techne Brand

Citations (3)

Product Datasheet / COA / SDS

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Knockout Validated, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Product Summary for Human/Mouse HSP27 Antibody

Immunogen

E. coli-derived recombinant human HSP27
Accession # P04792

Specificity

Detects recombinant and endogenous human and mouse HSP27 in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse HSP27 Antibody

Detection of Human/Mouse HSP27 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, HeLa human cervical epithelial carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse HSP27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1580) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HSP27 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of Human HSP27 by Simple Western^TM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP27 at approximately 31 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human/Mouse HSP27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1580). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Mouse HSP27 by Simple Western^TM.

Simple Western lane view shows lysates of C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP27 at approximately 31 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human/Mouse HSP27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1580). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Applications for Human/Mouse HSP27 Antibody

Application

Recommended Usage

Knockout Validated

HSP27 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in HSP27 knockout HeLa cell line.

Simple Western

0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line

Western Blot

0.1 µg/mL
Sample: MCF-7 human breast cancer cell line, HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblase cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HSP27

Heat shock proteins (HSPs) are a family of highly conserved stress response proteins. Heat shock proteins function primarily as molecular chaperones by facilitating the folding of other cellular proteins, preventing protein aggregation or targeting improperly folded proteins to specific degradative pathways. HSPs are typically expressed at low levels under normal physiological conditions but are dramatically up-regulated in response to cellular stress. Elevated levels of HSPs have been observed in association with ischemia/reperfusion, cancer, and chronic heart failure. HSP27, also known as HSPB1, is a member of the small heat shock protein family, which also includes HSP25 and the alpha-crystallins. HSP27 forms a large oligomer and the extent of phosphorylation plays a role in determining specific functions. HSP27 also functions as an anti-apoptotic molecule, regulating apoptosis through direct interaction with key components of the apoptotic pathway. HSP27 binds and sequesters cytochrome c released from the mitochondria in response to an apoptotic stimulus. This prevents the proper assembly of the apoptosome and subsequently, the activation of procaspase-9 and procaspase-3. Full length human HSP27 shares 83% and 81% aa identity with mouse and rat HSP27, respectively.

References

Gusev, N.B. et al. (2002) Biochemistry (Moscow) 67:511.
Garrido, C. et al. (2001) Biochem. Biophys. Res. Commun. 286:433.
Garrido, C. (2002) Cell Death Diffr. 9:483.
Brvey, J-M. et al. (2000) Nat. Cell Biol. 2:645.

Long Name

Heat Shock Protein 27

Alternate Names

HSP25, HSPB1

Entrez Gene IDs

3315 (Human); 15510 (Mouse); 24471 (Rat)

Gene Symbol

HSPB1

UniProt

P04792

Product Documents for Human/Mouse HSP27 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human/Mouse HSP27 Antibody

For research use only

Citations for Human/Mouse HSP27 Antibody (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse HSP27 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Banerjee S et al. (2011-01-11), Heat shock protein 27 differentiates tolerogenic macrophages that may support human breast cancer progression. Cancer Res., 2011-071(2):318-27.
PMID: 21224361
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Graner MW et al. (2007-10-17), The heat shock response and chaperones/heat shock proteins in brain tumors: surface expression, release, and possible immune consequences. J. Neurosci., 2007-127(42):11214-27.
PMID: 17942716
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Takai S et al. (2006-08-01), Phosphatidylinositol 3-kinase/Akt plays a role in sphingosine 1-phosphate-stimulated HSP27 induction in osteoblasts. J. Cell. Biochem., 2006-098(5):1249-56.
PMID: 16514645

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