Human/Mouse Frizzled-2 Antibody

Detection of Frizzled-2 by Western Blot

Small intestinal epithelial cells of Plcb3−/− mice exhibit downregulation of the Wnt/ beta-catenin signaling pathway. (A) GSEA indicates that the gene expression pattern in isolated IECs from Plcb3−/− mice correlates with the reduced expression of Wnt/ beta-catenin signature genes (upper left), Lgr4 regulated signature genes (lower left), Lgr4/Lgr5 regulated signature genes (upper right), and Ascl2 signature genes (lower right). NES: normalized enrichment score. n = 3 biological replicates. (B) qRT-PCR analysis of isolated IECs from WT and Plcb3−/− mice exposed to sterilized water or 3% DSS for 2 days. Normalized against Hprt mRNA. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001, determined by two-tailed Student’s t-test. (C) GSEA with isolated IECs from Plcb3 ∆IEC mice indicated the reduced expression of Wnt/ beta-catenin (upper left), Lgr4 (lower left), Lgr4/Lgr5 (upper right), and Ascl2 (lower right) signature genes at the homeostatic condition. (D) Small intestines of WT and Plcb3−/− mice exposed to sterilized water or 3% DSS for 2 days were separated into mesenchymal tissues. RNAs isolated were subjected to qRT-PCR analysis. mRNA amounts for several Wnt ligands and Wnt secretion enablers (Wls and Porcn) were normalized against Hprt. * p < 0.05; ** p < 0.01, determined by Student’s t-test. (E) Western blot analysis of Wnt/ beta-catenin signaling proteins from WT and Plcb3−/− mice (3 mice each) under H2O and 2-day DSS treatments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38891118), licensed under a CC-BY license. Not internally tested by R&D Systems.