Human/Mouse Cyclin D1/D2 Antibody

Name: Human/Mouse Cyclin D1/D2 Antibody
Brand: R&D Systems
SKU: AF4196
Price: 152 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF4196

Citations (6)

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Species Reactivity:	Human, Mouse
Immunogen:	E. coli-derived recombinant human Cyclin D1 Met1-Ile295 Accession # P24385
Applications:	CyTOF-ready, Flow Cytometry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human and mouse Cyclin D1/D2 in Western blots.

View Full Technical Details for Human/Mouse Cyclin D1/D2 Antibody

Applications for Human/Mouse Cyclin D1/D2 Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	2.5 µg/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human/Mouse Cyclin D1/D2 Antibody

	Western Blot Detection of Human/Mouse Cyclin D1/D2 by Western Blot. Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line, U2OS human osteosarcoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse Cyclin D1/D2 Cross-reactive Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4196) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human Cyclin D1, D2, and D3 (5 ng/lane) were included. A specific band for Cyclin D1 was detected at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Intracellular Staining by Flow Cytometry Detection of Cyclin D1/D2 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Goat Anti-Human/Mouse Cross-reactive Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4196, filled histogram) or isotype control AA175antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldeyde and permeabilized with saponin.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Cyclin D1/D2

The D-type cyclins (cyclins D1, D2, and D3) and their associated kinases, Cdk4 and Cdk6, play an important role in the progression from G₀/G₁ to S phase in the mammalian cell cycle. Cyclin D complexes containing Cdk4 or Cdk6 phosphorylate the retinoblastoma protein (pRb). pRb phosphorylation leads to the release of E2F transcription factors, which activate the expression of S-phase genes and thereby induce cell cycle progression. Cyclin D1, independent of Cdk4 activity, functions as a transcriptional modulator by regulating the activity of several transcription factors, such as estrogen receptor, Myb, and STAT3. Cyclin D1 has also been linked to the development and progression of several cancers including breast, bladder, esophagus, and lung. Overexpression of Cyclin D2 has been reported in gastric carcinoma, ovarian granulose cell tumors, and hematopoietic cell cancers, while in breast cancers, cyclin D2 expression is undetectable in 80% of the tumors.

Item	Value
Entrez Gene IDs	595 (Human); 12443 (Mouse); 58919 (Rat)
Alternate Names	BCL1;CCND1;D11S287E;G1/S-specific cyclin-D1;PRAD1;U21B31, Cyclin D1/D2

Citations for Human/Mouse Cyclin D1/D2 Antibody (6)

Showing 1 - 6 of 6 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse Cyclin D1/D2 Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

XF Liu et al. (2020-01-01), LncRNA SNHG6 regulating Hedgehog signaling pathway and affecting the biological function of gallbladder carcinoma cells through targeting miR-26b-5p Eur Rev Med Pharmacol Sci, 24(14):7598-7611.
PMID: 32744686
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

YJ Liu et al. (2018-01-01), MicroRNA-505 is downregulated in human osteosarcoma and regulates cell proliferation, migration and invasion Oncol. Rep., 39(2):491-500.
PMID: 29251324
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Q Chen et al. (2016-01-01), IGFBP6 is a novel nasopharyngeal carcinoma prognostic biomarker Oncotarget, 7(42):68140-68150.
PMID: 27623076
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

MacLeod R (2013-01-01), Extracellular calcium-sensing receptor/PTH knockout mice colons have increased Wnt/beta-catenin signaling, reduced non-canonical Wnt signaling, and increased susceptibility to azoxymethane-induced aberrant crypt foci. Lab Invest, 93(5):520-7.
PMID: 23545937
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Muruganandan S et al. (2011-01-01), Chemerin, a novel peroxisome proliferator-activated receptor gamma (PPARgamma) target gene that promotes mesenchymal stem cell adipogenesis. J. Biol. Chem., 286(27):23982-95.
PMID: 21572083
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Muruganandan S et al. (), Chemokine-Like Receptor 1 Is a Novel Wnt Target Gene that Regulates Mesenchymal Stem Cell Differentiation. Stem Cell35(3):711-724.
PMID: 27733019

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Showing 1 - 6 of 6 citations Showing All

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Human/Mouse Cyclin D1/D2 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human/Mouse Cyclin D1/D2 Antibody

Scientific Data Examples for Human/Mouse Cyclin D1/D2 Antibody

Western Blot

Intracellular Staining by Flow Cytometry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Cyclin D1/D2

Citations for Human/Mouse Cyclin D1/D2 Antibody (6)

Product Documents for Human/Mouse Cyclin D1/D2 Antibody

Product Datasheets

COA

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