Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC350F
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Immunohistochemistry, Immunohistochemistry-Frozen
Label
FITC (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 79018
Product Specifications
Immunogen
E. coli-derived recombinant human CXCL12/SDF-1 alpha
Lys22-Lys89
Accession # Q6ICW0
Lys22-Lys89
Accession # Q6ICW0
Specificity
Detects human and mouse CXCL12/SDF-1 alpha and CXCL12/SDF-1 beta in ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Antibody
Detection of CXCL12/SDF‑1 in MG‑63 Human Cell Line by Flow Cytometry.
MG-63 human osteosarcoma cell line was stained with Mouse Anti-Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Mono-clonal Antibody (Catalog # IC350F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CXCL12/SDF-1
CXCL12, also known as SDF-1, is a CXC chemokine that interacts with CXCR4. SDF-1 alpha and SDF-1 beta are alternatively spliced isoforms of CXCL12. SDF-1 beta has an additional 4 amino acids at the carboxy-terminus.
Alternate Names
PBSF, SDF-1, SDF1
Gene Symbol
CXCL12
UniProt
Additional CXCL12/SDF-1 Products
Product Documents for Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Antibody
Product Specific Notices for Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Antibody
For research use only
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