Human/Mouse CD44 PE‑conjugated Antibody

Catalog # FAB6127P | R&D Systems, Inc. a Bio-Techne Brand
Recombinant Monoclonal Antibody.
Catalog #
Availability
Size / Price
Qty
FAB6127P-025
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Recombinant Monoclonal Rat IgG2B Clone # IM7.8.1R

Product Summary for Human/Mouse CD44 PE‑conjugated Antibody

Immunogen

Myeloid leukemia M1 cells induced with Dexamethasone

Specificity

Detects human and mouse CD44 in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human/Mouse CD44 PE‑conjugated Antibody

Detection of CD44 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of CD44 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cell (PBMCs) were stained with Rat Anti-Human/Mouse CD44 PE-conjugated Monoclonal Antibody (Catalog # FAB6127P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CD44 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CD44 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Human/Mouse CD44 PE‑conjugated Monoclonal Antibody (Catalog # FAB6127P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Mouse CD44 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cell (PBMCs) and mouse splenocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using FAB6127P in the following applications:

Formulation, Preparation and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD44

CD44, also known as ECMR-III, Hermes Antigen, Pgp1, Ly-24, GP90, and HUTCH-I, is a ubiquitously expressed protein that is the major receptor for hyaluronic acid (HA). CD44 mediates cell-cell and cell-matrix interaction through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagen, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Mouse CD44 has a 22 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 48-463 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. CD44 exists as a large number of different isoforms due to alternative RNA splicing. Clone IM7.8.1 has been reported to recognize all isoforms of CD44. In the ECD, human and mouse CD44 share about 65% aa sequence identity.

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Entrez Gene IDs

960 (Human); 12505 (Mouse); 100126860 (Porcine); 25406 (Rat)

Gene Symbol

CD44

Product Documents for Human/Mouse CD44 PE‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse CD44 PE‑conjugated Antibody

For research use only

Citations for Human/Mouse CD44 PE‑conjugated Antibody (1)

Showing  1 - 1 of 1 citation Showing All
Filter By:
Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse CD44 PE‑conjugated Antibody include:

There are no citations that match your criteria.

Showing  1 - 1 of 1 citation Showing All

Customer Reviews for Human/Mouse CD44 PE‑conjugated Antibody (1)

5 out of 5
1 customer rating
5 stars
100%
4 stars
0%
3 stars
0%
2 stars
0%
1 star
0%

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
  • Human/Mouse CD44 PE-conjugated Antibody
    Name: Tahmineh Safaie
    Application: Flow Cytometry
    Sample Tested: 4T1 mouse breast cancer cell line
    Species: Mouse
    Verified Customer | Posted 11/08/2019
    Human/Mouse CD44 PE‑conjugated Antibody FAB6127P

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All
Setting the standard in quality research reagents