Human/Mouse Aurora B Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4006
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry-Paraffin, Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Aurora B
Met1-Ile150
Accession # Q96GD4
Met1-Ile150
Accession # Q96GD4
Specificity
Detects human and mouse Aurora B in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse Aurora B Antibody
Detection of Human Aurora B by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human Aurora B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4006) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Aurora B at approximately 40 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for Human/Mouse Aurora B Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and A20 mouse B cell lymphoma cell line
Sample: HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and A20 mouse B cell lymphoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aurora B
Aurora B, a serine/threonine protein kinase, is a key regulator of the onset of cytokinesis and chromosome segregation. Phosphorylation of Histone H3 at Ser 10 and Ser 28 by Aurora B contributes to chromosome number instability and mitotic chromosome condensation. In addition, Aurora B phosphorylates MCAK (mitotic centromere-associated kinesin) on Ser 92 and this inhibits MCAK’s microtubule depolymerizing activity. Aurora B localizes to kinetochore fibers during chromosome movement and segregation.
Alternate Names
Aik2, AIM-1, AIM1, ARK2, AURKB, IPL1, STK12, STK5
Gene Symbol
AURKB
UniProt
Additional Aurora B Products
Product Documents for Human/Mouse Aurora B Antibody
Product Specific Notices for Human/Mouse Aurora B Antibody
For research use only
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