Western blot shows lysates of HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human Aurora B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4006) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Aurora B at approximately 40 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human/Mouse Aurora B Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and A20 mouse B cell lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aurora B

Aurora B, a serine/threonine protein kinase, is a key regulator of the onset of cytokinesis and chromosome segregation. Phosphorylation of Histone H3 at Ser 10 and Ser 28 by Aurora B contributes to chromosome number instability and mitotic chromosome condensation. In addition, Aurora B phosphorylates MCAK (mitotic centromere-associated kinesin) on Ser 92 and this inhibits MCAK’s microtubule depolymerizing activity. Aurora B localizes to kinetochore fibers during chromosome movement and segregation.

Alternate Names

Aik2, AIM-1, AIM1, ARK2, AURKB, IPL1, STK12, STK5

Entrez Gene IDs

9212 (Human); 20877 (Mouse); 114592 (Rat)

Gene Symbol

AURKB

UniProt

Q96GD4

Additional Aurora B Products

Product Documents for Human/Mouse Aurora B Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse Aurora B Antibody

For research use only

Citations
Reviews

Human/Mouse Aurora B Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4006

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Aurora B Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse Aurora B Antibody

Detection of Human Aurora B by Western Blot.