Human MMP-9 Antibody

Name: Human MMP-9 Antibody
Brand: R&D Systems
SKU: MAB11756

R&D Systems, part of Bio-Techne | Catalog # MAB11756

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Product Datasheet / COA / SDS

Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human MMP‑9 Ala20-Asp707 (Gln279Arg) Accession # P14780
Applications:	Simple Western, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG Clone # 526501
Specificity:	Detects recombinant mouse MMP-9 protein in Direct ELISA.

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Technical Data
Citations / Reviews

Applications for Human MMP-9 Antibody

	Recommended Concentration	Sample
Western Blot	2 µg/mL	Human spleen
Simple Western	10-50 µg/mL	Human spleen

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human MMP-9 Antibody

	Western Blot Detection of Human MMP‑9 by Western Blot. Western Blot shows lysates of human spleen tissue. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human MMP‑9 Monoclonal Antibody (Catalog # MAB11756) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP‑9 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
	Simple Western Detection of Human MMP‑9 by Simple Western^TM. Left: Simple Western lane view shows lysates of human spleen, loaded at 0.5 mg/ml. A specific band was detected for MMP‑9 at approximately 150 kDa (as indicated) using both 10 µg/ml and 50 µg/ml of Mouse Anti-Human MMP‑9 Monoclonal Antibody (Catalog # MAB11756) followed by HRP-conjugated Goat Anti-Mouse Secondary Antibody (Catalog # 042-205). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Right: Simple Western electropherogram showing the same Mouse Anti-Human MMP‑9 Monoclonal Antibody (Catalog # MAB11756) tested at 10 µg/ml (blue line) and 50 µg/ml (green line) in the human spleen.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping	Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

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Background: MMP-9

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (Gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 maybe be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain.

Item	Value
Long Name	Matrix Metalloproteinase 9
Entrez Gene IDs	4318 (Human); 17395 (Mouse); 81687 (Rat); 102117693 (Cynomolgus Monkey)
Alternate Names	92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, EC 3.4.24, EC 3.4.24.35, GELB, Gelatinase B, MANDP2, MMP-9, MMP9, macrophage gelatinase, matrix metallopeptidase 9, matrix metalloproteinase 9, matrix metalloproteinase-9, type V collagenase

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Human MMP-9 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11756

Key Product Details

Applications for Human MMP-9 Antibody

Scientific Data Examples for Human MMP-9 Antibody

Western Blot

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: MMP-9

Customer Reviews for Human MMP-9 Antibody

Product Documents for Human MMP-9 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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