Human MMP-10 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB910
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 110304
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP-10
Tyr18-Cys476
Accession # P09238
Tyr18-Cys476
Accession # P09238
Specificity
Detects human MMP-10 in Western blots. Does not cross-react with recombinant human MMP-1, -2, -3, -7, -8, -9, -12, or -13.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human MMP-10 Antibody
MMP-10 in Human Colon Cancer Tissue.
MMP-10 was detected in immersion fixed paraffin-embedded sections of human colon cancer using Human MMP-10 Monoclonal Antibody (Catalog # MAB910) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human MMP-10 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Western Blot
1 µg/mL
Sample: Recombinant Human MMP-10 Western Blot Standard (Catalog # WBC026)
Sample: Recombinant Human MMP-10 Western Blot Standard (Catalog # WBC026)
Reviewed Applications
Read 2 reviews rated 3 using MAB910 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-10
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-10 (stromelysin 2) degrades a broad range of substrates including gelatin, collagen types III, IV and V, fibronectin, aggrecan, and pig cartilage proteoglycan. MMP-10 can activate other MMPs such as MMP-1 and MMP-8. MMP-10 is expressed in keratinocytes, T cells, menstrual endometrium, and a few tumor samples. Structurally, MMP-10 may be divided into four distinct domains: a pro-domain which is cleaved upon activation, a catalytic domain containing the zinc binding site; a short linker region, and a carboxyl terminal hemopexin-like domain.
Long Name
Matrix Metalloproteinase 10
Alternate Names
MMP10, Stromelysin 2
Entrez Gene IDs
4319 (Human)
Gene Symbol
MMP10
UniProt
Additional MMP-10 Products
Product Documents for Human MMP-10 Antibody
Product Specific Notices for Human MMP-10 Antibody
For research use only
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