MKP-3 was detected in immersion fixed PC-3 human prostate cancer cell line (left panel; positive stain) and K562 human chronic myelogenous leukemia cell line (right panel; negative stain) using Mouse Anti-Human MKP-3 Monoclonal Antibody (Catalog # MAB35761) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human MKP-3 Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed PC-3 human prostate cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MKP-3

MAP Kinase Phosphatase 3 (MKP-3), also called PYST1 and Dual Specificity Phosphatase 6 (DUSP6), dephosphorylates serine, threonine, and tyrosine residues in proteins. On Western blots, MKP-3 has an apparent molecular weight of 42 kilodaltons. It has a high degree of substrate selectivity for dephosphorylating ERK kinases over stress-related kinases such as p38 and JNK. MKP-3 is ubiquitinylated prior to degradation in the proteosome. The rate of MKP-3 degradation is enhanced by ERK-mediated phosphorylation.

Long Name

MAP Kinase Phosphatase 3

Alternate Names

DUSP6, MKP3, PYST1

Entrez Gene IDs

1848 (Human)

Gene Symbol

DUSP6

UniProt

Q16828

Additional MKP-3 Products

Product Documents for Human MKP-3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human MKP-3 Antibody

For research use only

Citations
Reviews

Human MKP-3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB35761

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all MKP-3 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human MKP-3 Antibody

MKP‑3 in PC-3 and K562 Human Cell Lines.