Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human MAD1L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4194) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MAD1L1 at approximately 90 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human MAD1L1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MAD1L1

MAD1L1 (mitotic arrest deficient 1-like 1) is a member of the spindle-assembly checkpoint (SAC) which prevents anaphase from occurring until all chromosomes are properly aligned at the metaphase plate. Upon checkpoint activation, MAD1L1 recruits MAD2 to unattached kinetochores, promoting the binding of MAD2 to CDC20, the activator for the anaphase-promoting complex. MAD1L1 has been implicated to have tumor-suppressing functions, but in small-cell lung cancer cell lines, the MAD1L1 gene is the most frequent copy number gain, suggesting a more complex role.

Long Name

MAD1 Mitotic Arrest Deficient-Like 1

Alternate Names

HsMAD1, MAD1, PIG9, TP53I9, TXBP181

Entrez Gene IDs

8379 (Human); 17120 (Mouse); 680006 (Rat)

Gene Symbol

MAD1L1

UniProt

Q9Y6D9

Additional MAD1L1 Products

Product Documents for Human MAD1L1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human MAD1L1 Antibody

For research use only

Citations
Reviews

Human MAD1L1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4194

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all MAD1L1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human MAD1L1 Antibody

Detection of Human MAD1L1 by Western Blot.