Human Ly6K Alexa Fluor® 488-conjugated Antibody

Name: Human Ly6K Alexa Fluor® 488-conjugated Antibody
Brand: R&D Systems
SKU: AF6648G

R&D Systems, part of Bio-Techne | Catalog # AF6648G

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Product Datasheet / COA

Key Product Details

Species Reactivity:	Human
Immunogen:	Chinese hamster ovary cell line CHO-derived recombinant human Ly6K Asp18-Gly138 Accession # Q17RY6
Applications:	Immunohistochemistry, Western Blot
Label:	Alexa Fluor 488 (Excitation=488 nm, Emission=515-545 nm)
Specificity:	Detects human Ly6K in Western blots.

View Full Technical Details for Human Ly6K Alexa Fluor® 488-conjugated Antibody

Technical Data
Citations / Reviews

Applications for Human Ly6K Alexa Fluor® 488-conjugated Antibody

	Recommended Concentration	Sample
Western Blot	Optimal dilution of this antibody should be experimentally determined.
Immunohistochemistry	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

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Background: Ly6K

Ly6K (Lymphocyte antigen 6 locus K; also CO16) is a 26-27 kDa member of the Ly-6 antigen family of molecules. It has restricted expression, being described in testis and skin. Ly6K is found in/on carcinomas, and is known to circulate in normal and tumor-patient blood. Mature human Ly6K is a 121 amino acid (aa) GPI-linked (presumed) glycoprotein. It is synthesized as a 165 aa preproprecursor that contains a 17 aa signal sequence, a 121 aa mature region (aa 18-138), and a 27 aa C‑terminal propeptide. There are two potential splice variants, one that shows a 48 aa substitution, and another that shows a 33 aa substitution for aa 73-165. It is not clear if mouse Ly6K is a true molecular ortholog of human Ly6K. In any event, over aa 18-138, human Ly6K shares 39% aa identity with mouse Ly6K.

Item	Value
Long Name	Lymphocyte Antigen 6 Complex Locus K
Entrez Gene IDs	54742 (Human); 76486 (Mouse)
Alternate Names	CO16, CT97, FLJ35226, HSJ001348, Ly6K, cancer/testis antigen 97, ly-6K, lymphocyte antigen 6 complex, locus K, lymphocyte antigen 6K

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