Human KLF4 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC3640A

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IC3640A
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Polyclonal Goat IgG

View all KLF4 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human KLF4
Ala2-Phe470
Accession # AAH29923

Specificity

Detects human KLF4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately 50% cross‑reactivity with recombinant mouse (rm) KLF4 and less than 1% cross-reactivity with recombinant human (rh) KLF5, rhKLF6, and rmKLF15.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human KLF4 APC-conjugated Antibody

Detection of KLF4 antibody in BG01V Human Cells antibody by Flow Cytometry.

Detection of KLF4 in BG01V Human Cells by Flow Cytometry.

BG01V human embryonic stem cells were stained with Goat Anti-Human KLF4 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC3640A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human KLF4 APC-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: BG01V human embryonic stem cells fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

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Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: KLF4

Human KLF4, also known as Epithelial Zinc Finger protein (EZF), is a 53 kDa (470 aa) member of the kruppel C2H2-type zinc finger protein family. It contains three C2H2-type zinc fingers at the carboxyl terminus that preferentially bind to cis-DNA elements that are GC rich. KLF4 regulates the expression of target genes that are involved in different cellular functions. KLF4 is highly expressed in the epithelial cells of the skin and the gastrointestinal tract. Human and mouse KLF4 share 90% amino acid sequence identity.

Long Name

Kruppel-Like Factor 4

Alternate Names

EZF

Entrez Gene IDs

9314 (Human)

Gene Symbol

KLF4

UniProt

AAH29923

Additional KLF4 Products

Product Documents for Human KLF4 APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human KLF4 APC-conjugated Antibody

For research use only

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