Human KIR2DS1/CD158h PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB8887P

Recombinant Monoclonal Antibody.
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FAB8887P-025
FAB8887P-100
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1127B

View all KIR2DS1/CD158h Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with human KIR2DS1/CD158h
Met1-His245
Accession # Q14954

Specificity

Detects human KIR2DS1/CD158h in flow cytometry. Clone 1127B recognizes KIR2DS1 and some alleles of KIR2DL1, both members of the killer cell immunoglobulin-like receptor (KIR) family. Because Clone 1127B displays partial cross-reactivity with KIR2DL1, co-staining with Mouse Anti-Human KIR2DL1 Clone 143211 (Catalog # FAB1844A) is recommended.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human KIR2DS1/CD158h PE-conjugated Antibody

Detection of KIR2DS1/CD158h antibody in Human PBMCs antibody by Flow Cytometry.

Detection of KIR2DS1/CD158h in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Rabbit Anti-Human KIR2DS1/CD158h PE-conjugated Monoclonal Antibody (Catalog # FAB8887P) and either (A) Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) or (B) Mouse Anti-Human KIR2DL1/KIR2DS5 APC-conjugated Monoclonal Antibody (Catalog # FAB1844A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human KIR2DS1/CD158h PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: KIR2DS1/CD158h

The KIRs comprise a family of 14 polymorphic and homologous activating and inhibitory receptors expressed primarily on CD56dim NK cells. KIR2DS1 is an activating receptor with high homology to the inhibitory receptor KIR2DL1. Both KIR2DL1 and KIR2DS1 bind to HLA-C alleles containing the C2 epitope. KIR2DS1 and KIR2DL1 expression regulates NK cell licensing and activation in a number of immune contexts. Over amino acids (aa) 1-245, human KIR2DS1 shares 97% aa sequence identity with human KIR2DL1.

Long Name

Killer Cell Immunoglobulin-like Receptor, Two Domains, Short Cytoplasmic Tail, 1

Alternate Names

CD158h, EB6ActI

Entrez Gene IDs

3806 (Human)

Gene Symbol

KIR2DS1

UniProt

Q14954

Additional KIR2DS1/CD158h Products

Product Documents for Human KIR2DS1/CD158h PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human KIR2DS1/CD158h PE-conjugated Antibody

For research use only

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