Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Kell Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kell
aa 68-732

Specificity

Detects human Kell in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse Kell is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Kell Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human whole blood

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Kell, see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Kell

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kell

Kell, a type II membrane glycoprotein, is linked through a single disulfide bond to XK, a putative membrane transporter. The two proteins constitute the Kell blood group antigens (1). Kell is a zinc endopeptidase of the neprilysin (NEP) family which also includes endothelin converting enzymes (ECE-1 and ECE-2), PEX, XCE, DINE and several NEP-like proteins (2). It has been shown to cleave big endothelin-3 (ET-3) at Trp21-Ile22, yielding ET-3, and to a much lesser extent, big ET-1 and big ET-2 at Trp21-Val22, yielding ET-1 and ET-2 (3). Several different molecular defects cause the Kell null phenotype, which has no obvious clinical outcome (4).

References

  1. Lee, S. et al. (2000) Transfus. Med. Rev. 14:93.
  2. Turner, A.J. et al. (2001) BioEssays. 23:261.
  3. Lee, S. et al. (1999) Blood 94:1440.
  4. Lee, S. et al. (2001) J. Biol. Chem. 276:27281.

Long Name

Zinc protease blood group antigen

Alternate Names

CD238, KEL

Entrez Gene IDs

3792 (Human); 23925 (Mouse)

Gene Symbol

KEL

Additional Kell Products

Product Documents for Human Kell Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Kell Antibody

For research use only

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