Detects human Kallikrein 12 in direct ELISAs and Western blots. No cross-reactivity with recombinant human (rh) Kallikrein 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 14, 15, rhKallikrein beta1, rhHGF Activator, rhFactor VII, rhFactor X, rhFactor XI, rhThrombin or rhuPA-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human Kallikrein 12 Antibody

Neutralization of Kallikrein 12 Activity by Human Kallikrein 12 Antibody.

The cleavage of Boc-VPR-AMC (0.1 mM, Catalog # ES011) by Recombinant Human Kallikrein 12 (0.1 µg/mL, Catalog # 3095-SE) is measured after preincubation with increasing concentrations of Human Kallikrein 12 Monoclonal Antibody (Catalog # MAB40341). The ND₅₀ is typically 0.1 µg/mL.

Applications for Human Kallikrein 12 Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Kallikrein 12 (Catalog # 3095-SE), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Kallikrein 12 (Catalog # 3095-SE) under non-reducing conditions only

Neutralization

Measured by its ability to neutralize Recombinant Human Kallikrein 12 (0.1 µg/mL, Catalog # 3095-SE) cleavage of the fluorogenic peptide substrate Boc-VPR-AMC (0.1 mM, Catalog # ES011). The Neutralization Dose (ND₅₀) is typically 0.1 µg/mL.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 12

Human tissue Kallikrein 12, encoded by the KLK12 gene, is a secreted serine protease that belongs to the human tissue kallikrein family (1). It is present in many tissues, such as salivary gland, stomach and breast. KLK12 displays trypsin-like enzymatic activity. This activity can be inhibited by Serpin F2 (R&D Systems, Catalog # 1470-PI). The physiological functions of KLK12 still remain unclear. Its expression is modulated by steroid hormones and is down‑regulated in breast cancer (2). Human KLK12 has three splice variants resulting from alternative splicing of the 3’ end. The amino acid sequence of human KLK12 is 80%, 77%, 71% and 66% to that of bovine, canine, mouse, and rat.

References

Yousef, G.M. et al. (2000) Genomics. 69:331.
Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.

Alternate Names

KLK-L5, KLK12

Entrez Gene IDs

43849 (Human)

Gene Symbol

KLK12

UniProt

Q9UKR0

Additional Kallikrein 12 Products

Product Documents for Human Kallikrein 12 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Kallikrein 12 Antibody

For research use only

Citations
Reviews

Human Kallikrein 12 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB40341

Key Product Details

Species Reactivity

Validated:

Cited:

Applications