Human ITM2A Alexa Fluor™ Plus 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4876AFP488
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human ITM2A
Specificity
Detects human ITM2A in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) ITM2B is observed, and less than 1% cross-reactivity with rhITM2C is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human ITM2A Alexa Fluor™ Plus 488-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: ITM2A
ITM2A (Integral membrane protein 2A; also protein E25) is a 43-45 kDa member of the ITM2 family of proteins. It is expressed by chondrocytes involved in endochondrial ossification, skeletal muscle, adipose tissue-derived stem cells, and CD3-activated CD4+ and CD8+ T cells. ITM2A is found embedded in both plasma membrane and Golgi complex. Human ITM2A is a 263 amino acid (aa) type II transmembrane glycoprotein. The ECD (aa 75-263) contains one BRICHOS domain (aa 133‑227). Proteolytic cleavage by furin is typical for BRICHOS domain-containing proteins. ITM2A may exist as a dimer. There are two potential splice variants, one that shows a deletion of aa 55-81, and a second that shows a deletion of aa 38-81. Full-length human ITM2A shares 95% aa identity with mouse ITM2A.
Long Name
Integral Membrane Protein 2A
Alternate Names
BRICD2A, E25A
Gene Symbol
ITM2A
UniProt
Additional ITM2A Products
Product Specific Notices for Human ITM2A Alexa Fluor™ Plus 488-conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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