Human IRF3 Alexa Fluor® 488-conjugated Antibody

Name: Human IRF3 Alexa Fluor® 488-conjugated Antibody
Brand: R&D Systems
SKU: IC4019G

R&D Systems, Inc. a Bio-Techne Brand

Catalog # IC4019G

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Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human IRF3 aa 206-427 Accession # Q14653
Applications:	Flow Cytometry
Label:	Alexa Fluor 488 (Excitation=488 nm, Emission=515-545 nm)
Source:	Monoclonal Mouse IgG_2B Clone # 482205
Specificity:	Detects human IRF3 in Western blots.

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Applications for Human IRF3 Alexa Fluor® 488-conjugated Antibody

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	5 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human IRF3 Alexa Fluor® 488-conjugated Antibody

	Intracellular Staining by Flow Cytometry Detection of IRF3 in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human IRF3 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC4019G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: IRF3

IRF3 (interferon response factor 3) is a 60 kDa member of the IRF family of proteins. Human IRF3 contains one DNA binding domain (aa 7‑107), a nuclear export signal (aa 139‑149) and multiple phosphorylation sites (aa 395‑407). Viral infection stimulates IRF3 phosphorylation, nuclear translocation and stimulation of IFN production. Alternate splice forms may exist. One will show a deletion of aa 201‑327, a second will show the same deletion plus an alternate start site at Met147, and a third will show a 125 aa substitution for the C-terminal 100 aa (aa 328‑427). Over aa 206‑427, human IRF3 is 76% and 83% aa identical to mouse and pig IRF3, respectively.

Item	Value
Long Name	Interferon Regulatory Factor 3
Entrez Gene IDs	3661 (Human); 54131 (Mouse)
Alternate Names	IRF-3, IRF3, interferon regulatory factor 3

Product Specific Notices for Human IRF3 Alexa Fluor® 488-conjugated Antibody

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citations for Human IRF3 Alexa Fluor® 488-conjugated Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human IRF3 Alexa Fluor® 488-conjugated Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

P Deb et al. (2020), Triggering of the cGAS-STING Pathway in Human Plasmacytoid Dendritic Cells Inhibits TLR9-Mediated IFN Production J. Immunol., 205(1):223-236.
PMID: 32471881

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Human IRF3 Alexa Fluor® 488-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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