Human Integrin beta8 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB47752
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2723C
Product Specifications
Immunogen
Chinese Hamster Ovary cell line CHO-derived human Integrin alpha V beta 8
Human Integrin alphaV (Phe31-Val992) and Human Integrin beta8 (Glu43-Arg684)
Accession # NP_002201.1
Human Integrin alphaV (Phe31-Val992) and Human Integrin beta8 (Glu43-Arg684)
Accession # NP_002201.1
Specificity
Detects human Integrin beta8 in direct ELISAs
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Integrin beta8 Antibody
Detection of Integrin beta 8 in MG-63 Human Cell Line by Flow Cytometry.
MG-63 human osteosarcoma cell line was stained with Rabbit Anti-Human Integrin beta 8 Monoclonal Antibody (Catalog # MAB2723C, filled histogram) or Rabbit IgG Control Antibody (MAB1050,open histogram) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). Staining was performed using our Staining Membrane-associated Proteins protocol.
Integrin beta8 in MG-63 Human Cell Line.
Integrin beta8 was detected in immersion fixed MG-63 human osteosarcoma cell line using Rabbit Anti-Human Integrin beta8 Monoclonal Antibody (Catalog # MAB47752) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human Integrin beta8 Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: MG‑63 human osteosarcoma cell line
Sample: MG‑63 human osteosarcoma cell line
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed MG‑63 human osteosarcoma cell line
Sample: Immersion fixed MG‑63 human osteosarcoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 8
Integrin beta 8 (Integrin beta8) is a 90 kDa type I transmembrane glycoprotein of the Integrin family of adhesion molecules. It associates with Integrin alphaV to form a receptor for vitronectin, fibrin, and the latency associated peptide (LAP). Binding to LAP promotes the proteolytic release of active TGF-beta from LAP. Integrin alphaV beta8 is required for vascular morphogenesis in the embryonic brain and yolk sac. Within the extracellular domain, human Integrin beta8 shares 87% aa sequence identity with mouse and rat Integrin beta8.
Alternate Names
ITGB8
Gene Symbol
ITGB8
UniProt
Additional Integrin beta 8 Products
Product Documents for Human Integrin beta8 Antibody
Product Specific Notices for Human Integrin beta8 Antibody
For research use only
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