Human IL-9R Antibody
R&D Systems, part of Bio-Techne | Catalog # AF290
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-9 R
Val40-Pro270
Accession # Q01113
Val40-Pro270
Accession # Q01113
Specificity
Detects human IL-9 R in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant human (rh) IL-2 sR gamma, rhIL-5 sR alpha, and rhIL-6 sR is observed and 10% cross-reactivity with rhIL‑2 sR beta is observed and 5% cross‑reactivity with rhIL-1 sRII, rhIL-4 sR, and rhIL-10 sR is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human IL-9R Antibody
Detection of IL-9R by Western Blot
Ucma/GRP−/− mVSMCs show increased expression of osteo/chondrogenic markers. Cells were treated with control (CM) or osteogenic medium (OM, with 2.6 mM phosphate) for 12 days. Panels (A–D) qPCR analysis of osteo/chondrogenic gene expression, n = 4–7. Statistical significance was assessed using t-test (Osteocalcin) or ANOVA (E) Western blotting analysis of osteo/chondrogenic markers. Both WT and KO cells were treated with OM. Figure shows full western blots obtained by cutting of a full-sized membrane prior to antibody incubations. Numbers in the middle show molecular weights of the protein standard. Numbers on the right show the expected molecular weights of the proteins. Panels F–I quantification of western blotting results from panel E, n = 3. Statistical significance was assessed using t-test. All graphs show mean + SD. *p between 0.05–0.01, **p between 0.001–0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29563538), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human IL-9R Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human IL-9 R Subunit (Catalog # 290-RNS)
Sample: Recombinant Human IL-9 R Subunit (Catalog # 290-RNS)
Reviewed Applications
Read 1 review rated 3 using AF290 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-9R
The specific high affinity functional IL-9 receptor complex consists of a ligand-specific IL-9 R( alpha) type I transmembrane subunit and the common gamma chain that is shared with the receptors for IL-2, IL-4, IL-7 and IL-15. Both the IL-9 R subunit and the common gamma chain are members of the hematopoietin receptor superfamily. Human and mouse IL-9 receptors share 53% amino acid sequence identity, and contain 468 and 533 amino acids, respectively. As a result of alternative splicing, cDNA clones encoding isoforms of IL-9 R, including a putative soluble form, have also been identified. Cells known to express IL-9 R include T cells, neutrophils, mast cells and macrophages.
Long Name
Interleukin 9 Receptor
Alternate Names
CD129, IL-9 R, IL9R
Gene Symbol
IL9R
UniProt
Additional IL-9R Products
Product Documents for Human IL-9R Antibody
Product Specific Notices for Human IL-9R Antibody
For research use only
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