Human IL-36 gamma/IL-1F9 Alexa Fluor® 350-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB2320U
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Blockade of Receptor-ligand Interaction, Western Blot
Label
Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 278706
Product Specifications
Immunogen
E. coli-derived recombinant human IL‑36γ/IL‑1F9
Met1-Asp169
Accession # Q9NZH8
Met1-Asp169
Accession # Q9NZH8
Specificity
Detects human IL‑36 gamma/IL‑1F9 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-36Ra/IL-1F5, rhIL-36 alpha/IL-1F6, rhIL-1F7, rhIL-36 beta/IL-1F8, rhIL-1ra/IL-1F3, or rhIL-18/IL-1F4 is
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: IL-36 gamma/IL-1F9
Interleukin-36 gamma (IL-36 gamma) [also named IL-1F9, IL-1 epsilon (epsilon), and IL-1H1] is a member of the IL-1 family, which includes IL-1 beta, IL-1 alpha, IL-1ra, IL-18 and IL-1F5 through F10 (1‑4). All family members show a 12 beta-strand, beta-trefoil configuration, and are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). IL-36 gamma is synthesized as a 19 kDa, 169 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site (1, 2, 5). The molecule is secreted when transfected into 293T cells (5). Human to mouse, IL-36 gamma has 53% aa sequence identity. Within the family, IL-36 gamma shares 30%, 23%, 33%, 57%, 35%, 45%, and 32% aa sequence identity with IL-1 ra, IL-1 beta, IL-1F5, F6, F7, F8, and F10, respectively. Cells reported to express IL-36 gamma include Langerhans cells, keratinocytes/stratified squamous epithelium, plus chief cells and parietal cells of the stomach (4, 5). The receptor for IL-36 gamma is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (6). Recombinant IL-36 gamma, along with IL-36 beta/IL-1F8 and IL-36 alpha/IL-1F6, has been shown to activate the pathway involving NF-kappa B and MAPK in an IL-1 Rrp2 dependent manner.
Long Name
Interleukin 36 gamma/Interleukin 1 Family 9
Alternate Names
IL-1 H1, IL-1rp2, IL1F9, IL36 gamma, IL36G
Gene Symbol
IL36G
UniProt
Additional IL-36 gamma/IL-1F9 Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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