Human IL-21R Antibody

Name: Human IL-21R Antibody
Brand: R&D Systems
SKU: AF991

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF991

Citations (6)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Species Reactivity:	Human
Immunogen:	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑21 R Subunit Cys20-Pro236 Accession # Q9HBE5
Applications:	CyTOF-ready, Flow Cytometry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human IL‑21 R Subunit in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse IL‑21 R is observed.

View Full Technical Details for Human IL-21R Antibody

Applications for Human IL-21R Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human IL‑21 R Subunit Fc Chimera (Catalog # 991-R2)
Flow Cytometry	2.5 µg/10⁶ cells	Human whole blood CD19⁺ B cells
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-21R

Interleukin-21 (IL-21) and its receptor play an important role in the regulation of the immune system. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta, IL-4 R alpha and IL-9 R. The gene for human IL-21 R has been mapped to chromosome 16p12. Human IL-21 R is a 538 amino acid (aa) residue type I transmembrane protein with a 19 aa signal peptide, a 217 aa extracellular domain, a 19 aa transmembrane domain and a 283 aa cytoplasmic domain. IL-21 R is expressed on lymphoid tissues, peripheral B cells and cell lines of T, B and NK lineage. The common gamma chain ( gamma _c) is required for IL-21 R signaling. The IL-21/IL-21 R interaction appears to play an important role in B and T cell proliferation after antigen stimulation and NK cell maturation.

Item	Value
References	Parrish-Novak, et al. (2000) Nature 408:57. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144. Asao, H. et al. (2001) J. Immunol. 167:1.
Long Name	Interleukin 21 Receptor
Entrez Gene IDs	50615 (Human); 60504 (Mouse); 102135042 (Cynomolgus Monkey); 705759 (Rhesus Macaque)
Alternate Names	CD360, CD360 antigen, IL-21 R, IL-21 receptor, IL-21R, IL21R, MGC10967, NILR, Novel interleukin receptor, interleukin 21 receptor, interleukin-21 receptor

Citations for Human IL-21R Antibody (6)

Showing 1 - 6 of 6 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Human IL-21R Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Functional Assay

Kaltenmeier C et al. (2015), CD4+ T cell-derived IL-21 and deprivation of CD40 signaling favor the in vivo development of granzyme B-expressing regulatory B cells in HIV patients. J Immunol, 194(8):3768-77.
PMID: 25780036
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

de Totero D et al. (2010), Heterogeneous expression and function of IL-21R and susceptibility to IL-21-mediated apoptosis in follicular lymphoma cells. Exp. Hematol., 38(5):373-83.
PMID: 20193734
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Ansen S et al. (2008), Dissociation of its opposing immunologic effects is critical for the optimization of antitumor CD8+ T-cell responses induced by interleukin 21. Clin. Cancer Res., 14(19):6125-36.
PMID: 18829491
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Caruso R et al. (2007), IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis. J. Immunol., 178(9):5957-65.
PMID: 17442980
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Baan CC et al. (2007), Interleukin-21: an interleukin-2 dependent player in rejection processes. Transplantation, 83(11):1485-92.
PMID: 17565322
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Jungel A et al. (2004), Expression of interleukin-21 receptor, but not interleukin-21, in synovial fibroblasts and synovial macrophages of patients with rheumatoid arthritis. Arthritis Rheum., 50(5):1468-76.
PMID: 15146416

There are no citations that match your criteria.

Showing 1 - 6 of 6 citations Showing All

Product Documents for Human IL-21R Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Human IL-21R Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased