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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor 532 (Excitation = 534 nm, Emission = 553 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 473143

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-11 Rα
Cys26-Val363
Accession # Q14626

Specificity

Detects human IL-11 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with rmIL-11 R alpha or recombinant human IL‑6 R alpha is observed. In Western blots, 25% cross-reactivity with recombinant mouse (rm) IL-11 R alpha is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: IL-11 R alpha

Human interleukin 11 receptor alpha (IL-11 R alpha) is 150 kDa type I transmembrane protein in the hematopoietin cytokine receptor family that was cloned on the basis of its conserved WSXWS motif. IL-11 R alpha cDNA encodes a 422 amino acid (aa) residue precursor type I membrane protein, containg a signal peptide followed by extracellular (ECD), transmembrane and cytoplasmic domains. The extracellular region which is closely related to that of IL-6 R alpha and CNTF R alpha contains an immunoglobulin-like domain and two fibronectin-type III-like domains. Within the ECD, human IL-11 R alpha shares 85% and 83% aa sequence identity with mouse and rat IL-11 R alpha, respectively (1‑3). Alternate splicing generates an isoform of human IL-11 R alpha which lacks the cytoplasmic domain. By itself, IL-11 R alpha binds IL-11 with low affinity. IL-11 R alpha, together with gp130, forms a functional high-affinity receptor complex for IL-11. The expression of IL-11 R alpha has been detected in all adult tissues examined (lung, stomach, intestine), during embryonic development and in totipotent and differentiating embryonic stem cells. Recombinant soluble IL-11 R alpha has been shown to mediate IL-11 responsiveness in cells expressing the gp130 molecule. In cells expressing transmembrane IL-11 R alpha and gp130, soluble IL-11 R alpha has been shown to act as an IL-11 antagonist (3‑6).

Long Name

Interleukin 11 Receptor alpha

Alternate Names

IL-11Ra, IL11R alpha, IL11RA

Entrez Gene IDs

3590 (Human); 16157 (Mouse)

Gene Symbol

IL11RA

UniProt

Additional IL-11 R alpha Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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