Human IFN-beta Antibody

Name: Human IFN-beta Antibody
Brand: R&D Systems
SKU: AF814

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF814

Citations (7)

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Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human IFN-beta
Applications:	Block/Neutralize, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human IFN-beta in direct ELISAs and Western blots.

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Applications for Human IFN-beta Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human IFN-beta

Item	Value
Neutralization	Measured by its ability to neutralize IFN‑ beta inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND₅₀) is typically 0.100 - 1.00 µg/mL in the presence of 50 pg/mL Recombinant Human IFN‑ beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human IFN-beta Antibody

	Neutralization IFN‑ beta Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN-beta Antibody. Recombinant Human IFN-beta (8499-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN-beta (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IFN-beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF814). The ND₅₀ is typically 0.100 - 1.00 µg/mL.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IFN-beta

IFN-beta (interferon beta; also fibroblast IFN) is a secreted, monomeric 23-24 kDa member of the alpha/beta interferon family of molecules. It can be produced by almost all cell types in response to bacterial DNA or viral double-stranded RNA and is abundantly secreted by circulating plasmacytoid DCs. IFN-beta drives monocytic transformation into DCs, and it appears to stimulate a B cell switch from from IgM to IgG secretion. Mature human IFN-beta is 166 amino acids (aa) in length (aa 22-187) and contains one phosphorylation site at Ser140. There is one potential alternative start site at Met22. Full-length human IFN-beta (aa 22-187) shares 47% aa identity with mouse IFN-beta.

Item	Value
Long Name	Interferon beta
Entrez Gene IDs	3456 (Human); 15977 (Mouse); 24481 (Rat)
Alternate Names	Fibroblast interferon, IFB, IFBIFNB, IFF, IFN-beta, IFNB, IFNB1, IFNbeta, MGC96956, interferon beta, interferon, beta 1, fibroblast

Product Specific Notices for Human IFN-beta Antibody

Sold under license from Pestka Biomedical Laboratories, Inc. d/b/a PBL Interferon Source. For research use only. Not for resale. Not for diagnostic, therapeutic or clinical purposes.

Citations for Human IFN-beta Antibody (7)

Showing 1 - 7 of 7 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human IFN-beta Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Neutralization

LE Langbein et al. (2022), Data supporting the roles of BAP1, STING, and IFN-beta in ISGF3 activation in ccRCC Data in Brief, 45(0):108743.
PMID: 36426057
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

D Olmeda et al. (2021), Live imaging of neolymphangiogenesis identifies acute antimetastatic roles of dsRNA mimics Embo Molecular Medicine, 0(0):e12924.
PMID: 34762341
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Popugaeva E et al. (2012), Dobrava-Belgrade hantavirus from Germany shows receptor usage and innate immunity induction consistent with the pathogenicity of the virus in humans. PLoS ONE, 7(4):e35587.
PMID: 22545121
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Knoblach T et al. (2011), Human cytomegalovirus IE1 protein elicits a type II interferon-like host cell response that depends on activated STAT1 but not interferon-gamma. PLoS Pathog., 7(4):e1002016.
PMID: 21533215
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Rascle A et al. (2009), The LIM-homeodomain transcription factor LMX1B regulates expression of NF-kappa B target genes. Exp. Cell Res., 315(1):76-96.
PMID: 18996370
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Salaun B et al. (2006), TLR3 can directly trigger apoptosis in human cancer cells. J. Immunol., 176(8):4894-901.
PMID: 16585585
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Emori Y et al. (2004), Inhibition of human immunodeficiency virus type 1 replication by Z-100, an immunomodulator extracted from human-type tubercle bacilli, in macrophages. J. Gen. Virol., 85(0):2603-13.
PMID: 15302954

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Showing 1 - 7 of 7 citations Showing All

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Human IFN-beta Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human IFN-beta Antibody

Scientific Data Examples for Human IFN-beta Antibody

Neutralization