Human IFN-beta Antibody
R&D Systems, part of Bio-Techne | Catalog # 21400-1
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Neutralization
Cited:
Western Blot, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # MMHB-3
Product Specifications
Immunogen
Human interferon beta
Specificity
Binds to human interferon beta with high affinity. For best results in experiments involving neutralization of
the anti-viral effects of human interferon beta we recommend using Sheep Polyclonal Antibody to human interferon beta
(Product 31400-1).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human IFN-beta Antibody
Application
Recommended Usage
Neutralization
One neutralization unit is the amount of antiserum required to neutralize one unit of
human interferon beta (Hu-IFN-
beta) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition
assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981)
"Convenient Assay for Interferons," J. Virol. 37,
755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition
Assay for Interferon," in
Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this
antiviral assay for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%.
The units are determined with respect to the international reference standard for Hu-IFN-
beta provided by the National
Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in
Methods in Enzymology
(S. Pestka, ed.), Academic Press, New York 119, 14-23].
Due to the variation in ND50 (Neutralizing Dose) values based on the cell type and assay system, we recommend each user determine the neutralizing concentration of this antibody lot in their assay system. Using an A549/EMCV (cell/virus) system, we have not verified with reasonable consistency the neutralizing concentration of this antibody (the concentration required to inhibit the antiviral effect of human interferon beta by one half).
Due to the variation in ND50 (Neutralizing Dose) values based on the cell type and assay system, we recommend each user determine the neutralizing concentration of this antibody lot in their assay system. Using an A549/EMCV (cell/virus) system, we have not verified with reasonable consistency the neutralizing concentration of this antibody (the concentration required to inhibit the antiviral effect of human interferon beta by one half).
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Phosphate-buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
After receipt, the product may be stored at -20°C for short-term use (
≤ 6 months). For
long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the
contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing
should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers
containing protein such as 0.1% bovine serum albumin (BSA).
Background: IFN-beta
IFN-beta (Interferon-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.
Long Name
Interferon beta
Alternate Names
IFB, IFF, IFNB, IFNB1, IFNbeta
Gene Symbol
IFNB1
Additional IFN-beta Products
Product Documents for Human IFN-beta Antibody
Product Specific Notices for Human IFN-beta Antibody
For research use only
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