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Human IFN-beta Antibody

Bio-Techne includes R&D Systems | Catalog # MAB8144

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MAB8144-100
MAB8144-SP

Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 944403

Product Summary for Human IFN-beta Antibody

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human IFN‑ beta
Met1-Asn187
Accession # P01574

Specificity

Detects human IFN‑ beta in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human IFN-beta Antibody

Human IFN-beta  Antibody in ELISA Standard Curve.

Human IFN‑ beta ELISA Standard Curve.

Recombinant Human IFN-beta protein was serially diluted 2-fold and captured by Mouse Anti-Human IFN-beta Monoclonal Antibody (Catalog # MAB8144) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IFN-beta Monoclonal Antibody(Catalog # MAB8143) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human IFN-beta Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human IFN‑ beta Monoclonal Antibody (Catalog # MAB8143).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human IFN-beta DuoSet ELISA Kit (Catalog # DY814-05) for convenient development of a sandwich ELISA or the Human IFN-beta Quantikine ELISA Kit (Catalog # DIFNB0) for a complete optimized ELISA.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-beta

IFN-beta (interferon beta; also fibroblast IFN) is a secreted, monomeric 23-24 kDa member of the alpha/beta interferon family of molecules. It can be produced by almost all cell types in response to bacterial DNA or viral double-stranded RNA and is abundantly secreted by circulating plasmacytoid DCs. IFN-beta drives monocytic transformation into DCs, and it appears to stimulate a B cell switch from from IgM to IgG secretion. Mature human IFN-beta is 166 amino acids (aa) in length (aa 22-187) and contains one phosphorylation site at Ser140. There is one potential alternative start site at Met22. Full-length human IFN-beta (aa 22-187) shares 47% aa identity with mouse IFN-beta.

Long Name

Interferon beta

Alternate Names

IFB, IFF, IFNB, IFNB1, IFNbeta

Entrez Gene IDs

3456 (Human); 15977 (Mouse); 24481 (Rat)

Gene Symbol

IFNB1

UniProt

Additional IFN-beta Products

Product Documents for Human IFN-beta Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IFN-beta Antibody

For research use only

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