Human IFN-alpha Antibody

Name: Human IFN-alpha Antibody
Brand: R&D Systems
SKU: 31110-1

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 31110-1

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Species Reactivity:	Human
Immunogen:	Mixture of all recombinant human interferon alpha subtypes (IFNA2, IFNA8, IFNA10, IFNA1, IFNA21, IFNA5, IFNA14, IFNA17, IFNA7, IFNA6, IFNA4, IFNA16)
Applications:	Block/Neutralize
Label:	Unconjugated
Source:	Polyclonal Sheep Serum
Specificity:	Binds to and blocks biological activity of all human interferon alpha subtypes

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Applications for Human IFN-alpha Antibody

Neutralization

One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha
(Hu-IFN-alpha 2) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37, 755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN-alpha provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. This material is prepared specifically for effective neutralization of Hu-IFN-alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	N/A
Formulation	Neat serum diluted in phosphate buffered saline (PBS)
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	After receipt, the product may be stored at -20°C for short-term use (≤ 6 months). For long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).

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Background: IFN-alpha

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Item	Value
Long Name	Interferon alpha
Entrez Gene IDs	3440 (Human)
Alternate Names	IFN-alpha, IFN-alphaA, IFNA, IFNA2, IFNA2b, IFNalpha, INFA2, LeIF D, interferon alpha 2

Citations for Human IFN-alpha Antibody

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Human IFN-alpha Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human IFN-alpha Antibody

Formulation, Preparation and Storage

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Background: IFN-alpha

Citations for Human IFN-alpha Antibody

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