Detects human ICAM‑3/CD50 in ELISAs and Western blots. ICAM‑3/CD50 was found not to recognize COS cells expressing the humanICAM‑1 protein. The epitope for ICAM‑3/CD50 has been found to be located on domain 1, or the interface between domains 1 and 2 of the human ICAM‑3/CD50 molecule.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human ICAM-3/CD50 Antibody

ICAM‑3/CD50 in Human PBMCs.

ICAM-3/CD50 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human ICAM-3/CD50 Monoclonal Antibody (Catalog # BBA15) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Human ICAM-3 / CD50 ELISA Standard Curve

Recombinant Human ICAM-3/CD50 Fc Chimera (Catalog # 715-IC) was serially diluted and captured by Mouse Anti-Human ICAM-3/CD50 Monoclonal Antibody (Catalog # BBA15) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human ICAM-3/CD50 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF813) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human ICAM-3/CD50 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood monocytes

Immunocytochemistry

Cattoretti, G. et al. (1992) J. Pathol. 168:357.

Immunoprecipitation

Vazeux, R. et al. (1992) Nature 360:485.

Western Blot

1 µg/mL
Sample: Recombinant Human ICAM-3/CD50 Fc Chimera (Catalog # 715-IC)

Human ICAM-3/CD50 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Human ICAM-3/CD50 Biotinylated Antibody (Catalog # BAF813)
Standard: Recombinant Human ICAM-3/CD50 Fc Chimera Protein, CF (Catalog # 715-IC)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-3/CD50

Intercellular Adhesion Molecule-3 (ICAM-3, CD50), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and alpha_d/ beta₂. ICAM‑3 is expressed on leukocytes and epidermal Langerhans cells. It may play an important role in T cell stimulation by Langerhans cells. ICAM-3 has not been identified in the mouse.

References

Hayflick, J. et al. (1998) Immunologic Res. 17:313.

Long Name

Intercellular Adhesion Molecule 3

Alternate Names

CD50, ICAM3

Entrez Gene IDs

3385 (Human)

Gene Symbol

ICAM3

Additional ICAM-3/CD50 Products

Product Documents for Human ICAM-3/CD50 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ICAM-3/CD50 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human ICAM-3/CD50 Antibody

R&D Systems, part of Bio-Techne | Catalog # BBA15

Key Product Details

Species Reactivity

Validated:

Cited:

Applications