A172 human glioblastoma cell line was stained with Goat Anti-Human ICA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4084, filled histo-gram) or isotype control antibody (AB-108-C, open histo-gram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

ICA1 in paraffin-embedded human pancreas.

ICA1 was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human ICA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4084) at 0.5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (red; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to islets of Langerhans.

Applications for Human ICA1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

0.1 µg/mL
Sample: Recombinant Human ICA1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICA1

Islet Cell Autoantigen 1 (ICA1), also known as ICA 69 kDa (ICA69), is a cytosolic protein that is associated with the Golgi complex and with immature secretory granules. Its N-terminal region shares similarity with arfaptins (ADP-ribosylation factor-interacting-proteins), suggesting that ICA1 likely plays a role in membrane trafficking. Human ICA-1 is a 483 amino acids (aa) protein. As a result of aa insertions, deletions and substitutions, multiple isoforms exist. Human ICA-1 shows 89% and 94% aa identity to mouse and canine ICA-1, respectively.

Long Name

Islet Cell Autoantigen 1

Alternate Names

ICAp69

Entrez Gene IDs

3382 (Human)

Gene Symbol

ICA1

UniProt

ABW03414

Additional ICA1 Products

Product Documents for Human ICA1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ICA1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human ICA1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4084

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human ICA1 Antibody

Detection of ICA1 in A172 Human Cell Line by Flow Cytometry.