HLA DRB1 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human HLA DRB1 Monoclonal Antibody (Catalog # MAB11640) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human HLA-DRB1 Antibody

Application

Recommended Usage

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HLA-DRB1

HLA-DRB1 is an HLA class II histocompatibility antigen. It is part of a heterodimer consisting of an alpha (DRA) and a beta chain (DRB) which are both anchored in the membrane. Class II molecules are constitutively expressed in professional APC's. HLA-II is an important immune system protein that plays a role in tumor antigen presentation, immune response, and immune surveillance. HLA-DRB1 presents antigenic peptides to HLA-DRB1-restricted CD4+ T-cells. Aberrant expression of HLA-II is closely related to the pathogenesis of tumors.

References

"Entrez Gene: HLA-DRB1 major histocompatibility complex, Class II, DR beta I"
Hu JM, Li L, Chen YZ, Liu C, Cui X, Yin L, Yang L, Zou H, Pang L, Zhao J, Qi Y, Cao Y, Jiang J, Liang W, Li F. HLA-DRB1 and HLA-DQB1 methylation changes promote the occurrence and progression of Kazakh ESCC. Epigenetics. 2014 Oct;9(10):1366-73. doi: 10.4161/15592294.2014.969625. PMID: 25437052; PMCID: PMC4623353.
Zhao X, Li J, Zhu Q, Liang G, Xia W, He X, Zhu C, Qi H, Deng B, Chen X, Zhang J. HLA-A and HLA-DRB1 may play a unique role in ovarian teratoma-associated anti-N-methyl-D-aspartate receptor encephalitis. Reprod Biol Endocrinol. 2020 Nov 7;18(1):107. doi: 10.1186/s12958-020-00661-5. PMID: 33160385; PMCID: PMC7648266.

Long Name

Major histocompatibility complex, class II, DR beta 1

Alternate Names

DRB1, HLA-DR1B, HLA-DRB, SS1

Entrez Gene IDs

3123 (Human)

Gene Symbol

HLA-DRB1

UniProt

P01911 (Human)

Additional HLA-DRB1 Products

Product Documents for Human HLA-DRB1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human HLA-DRB1 Antibody

For research use only

Citations
Reviews

Human HLA-DRB1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11640

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all HLA-DRB1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human HLA-DRB1 Antibody

Detection of HLA DRB1 in Human Tonsil.