Human HGF Antibody

IL-11 Secretion Induced by HGF and Neutralization by Human HGF Antibody.

Recombinant Human HGF (Catalog # 294-HGN) induces IL-11 secretion in the Saos-2 human osteosarcoma cell line. The dose-dependent response (orange line) is measured by the Human IL-11 Duoset (Catalog # DY218). Under these conditions, IL-11 secretion elicited by HGF is neutralized (green line) by increasing concentrations of Goat Anti-Human HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-294-NA). The ND₅₀ is typically <2 µg/mL.

Detection of HGF by Immunohistochemistry

Extracellular HGF undergoes Y198/Y250 nitration during aging. (a–c) Direct‐immunofluorescence microscopy evidence of in vivo nitration. Lower hind limb muscles were collected from male S.D. rats of three age groups, young (2‐month‐old), adult (10‐month‐old), & old (20‐month‐old) (n = 3 each group). Serial cryo‐sections of calf muscle (mid‐belly portion, 13‐μm thickness) were blocked with sterile donkey serum/BSA/gelatin solution prior to incubation with Fluorescein‐labeled anti‐nitrated Y198‐HGF (clone 3A11C6; fluorescent false‐green) (a), HiLyte Fluor 647‐labeled anti‐nitrated Y250‐HGF (clone 6B82C3; fluorescent false‐yellow) (b), or Alexa Fluor 594‐labeled anti‐HGF alpha‐chain antibody (clone H‐10 from Santa Cruz Biotechnology; fluorescent false‐baby‐blue) (c). Sections were mounted in VECTASHIELD Antifade Mounting Medium & observed under a fluorescence microscope equipped with digital cameras & the Tile‐Scan program. Representative images of three rats per age group are shown here (see Figure S6 for other specimens). Gas, gastrocnemius; Pla, plantaris; Sol, soleus muscle. Lower row of each panel, high‐magnification views of boxed areas in an anterior‐side region of Gas lateral head (upper row, tiling images). (d) ECM‐bound HGF nitration monitored by WBting. Extraction of ECM‐associated HGF was conducted by 1.0 M NaCl wash of isolated satellite cells at 24‐h post‐plating (originally developed by Naldini et al., 1992) from the three age groups of rats (n = 3 rats per group) & subjected to WBting using anti‐nitrated Y198‐HGF mAb to monitor the nitration levels of extracellular HGF normalized to cell counts on the plates. The alpha‐chain & pro‐form of HGF are indicated by arrowheads. MW‐STD, MagicMark molecular weight standards; HGFnitro, nitrated recombinant HGF. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37985931), licensed under a CC-BY license. Not internally tested by R&D Systems.