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Human Hepsin Antibody

Catalog # MAB4776 | R&D Systems, Inc. a Bio-Techne Brand
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MAB4776
MAB4776-SP

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 506220

Product Summary for Human Hepsin Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Hepsin
Ile163-Leu417
Accession # P05981

Specificity

Detects human Hepsin in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Hepsin Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Hepsin (Catalog # 4776‑SE), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Hepsin (Catalog # 4776‑SE) under non-reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using MAB4776 in the following applications:

Published Applications

Read 1 publication using MAB4776 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hepsin

Hepsin, also known as TMPRSS1, is a Type II membrane protein with an extracellular serine protease domain (1). It is most highly expressed in liver, but is also present in many other tissues, notably lung, kidney, and skeletal muscle (2). A soluble form of Hepsin lacking the transmembrane domain has been identified (3). Hepsin is capable of activating Factor VII, and may initiate blood coagulation at the cell surface (4). Hepsin is overexpressed in various human tumors, including prostate (5), and is considered to be a biomarker for prostate cancer (6). rhHepsin was expressed as a secreted, soluble protein lacking its cytosolic and transmembrane domains. The D161E and R162K mutations were introduced into the prosequence to improve expression of the recombinant human Hepsin.

References

  1. Leytus, S.P. et al. (1988) Biochemistry 27:1067.
  2. Tsuji, A. et al. (1991) J. Biol. Chem. 266:16948.
  3. Li, Y. et al. (2005) Biomed. Biochim. Acta 1681:157.
  4. Kazama, Y. et al. (1995) J. Biol. Chem. 270:66.
  5. Dhanasekaran, S.M. et al. (2001) Nature 412:822.
  6. Wu, Q. and Parry, G. (2007) Front. Biosci. 12:5052.

Alternate Names

HPN, TMPRSS1

Entrez Gene IDs

3249 (Human); 15451 (Mouse); 29135 (Rat)

Gene Symbol

HPN

UniProt

Product Documents for Human Hepsin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Hepsin Antibody

For research use only

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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