GSK-3a was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human GSK-3a Monoclonal Antibody (Catalog # MAB2986) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human GSK-3 alpha Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GSK-3 alpha

Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified as an inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta) share 85% amino acid identity. GSK-3 alpha is inhibited by phosphorylation at S21 by Akt and other kinases. Deregulated GSK-3 has been implicated in several diseases including type II diabetes, Alzheimer’s disease, bipolar disorder, and cancer.

Long Name

Glycogen Synthase Kinase 3 alpha

Alternate Names

GSK3 alpha, GSK3A

Entrez Gene IDs

2931 (Human); 606496 (Mouse); 50686 (Rat)

Gene Symbol

GSK3A

UniProt

P49840.2

Additional GSK-3 alpha Products

Product Documents for Human GSK-3 alpha Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human GSK-3 alpha Antibody

For research use only

Citations
Reviews

Human GSK-3 alpha Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2986

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all GSK-3 alpha Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human GSK-3 alpha Antibody

GSK‑3 alpha in MCF‑7 Human Cell Line.