Human Granzyme H Alexa Fluor™ Plus 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB1377AFP647
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 185813
Product Specifications
Specificity
Detects human Granzyme H in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) Granzyme D or rmGranzyme G is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Background: Granzyme H
Granzyme H is a member of the granzyme family of serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme H’s functions are largely unknown. The more abundant expression of Granzyme H than Granzyme B in NK cells suggests that Granzyme H may complement the pro-apoptotic function of Granzyme B in this cell type (3). Human Granzyme H shows the highest amino acid identity (71%) to mouse Granzyme C (4). Human Granzyme H is synthesized as a precursor (246 residues) with a signal peptide (residues 1‑18), a propeptide (residues 19‑20) and a mature chain (residues 21‑246) (5‑7). After being activated by active cathepsin C, human Granzyme H cleaves a thioester substrate (8).
References
- Kam, C.-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
- Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
- Sedelies, K.A. et al. (2004) J. Biol. Chem. 279:26581.
- Sattar, R. et al. (2003) Biochem. Biophys. Res. Comm. 308:726.
- Meier, M. et al. (1990) Biochemistry 29:4042.
- Haddad, P. et al. (1991) Int. Immunol. 3:57.
- Klein, J.L. et al. (1990) Tissue Antigens 35:220.
- Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.
Alternate Names
CCP-X, CGL2, CTSGL2, GrzH, GZMH
Entrez Gene IDs
2999 (Human)
Gene Symbol
GZMH
UniProt
Additional Granzyme H Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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