Human GAPDH-2 Alexa Fluor® 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6276R
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human GAPDH-2
Glu75-Ala222
Accession # O14556
Glu75-Ala222
Accession # O14556
Specificity
Detects human GAPDH-2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human GAPDH and recombinant mouse GAPDH is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human GAPDH-2 Alexa Fluor® 647-conjugated Antibody
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: GAPDH-2
GAPDH-2 (Glyceraldehyde-3-phosphate dehydrogenase 2; also GAPD2 and GAPDS) is a 40-44 kDa member of the GAPDH family of enzymes. Due to a high incidence of proline residues, it may migrate anomalously at 56 kDa in SDS-PAGE. GAPDH-2 is expressed only in testis as a homotetramer, where it is found in the cytoplasm bound to the sperm fibrous sheath. Unlike GAPDH that has multiple functions, GAPDH-2 appears to participate only in carbohydrate metabolism, generating energy via glycolysis for sperm motility and fertility. Human GAPDH-2 is 408 amino acids (aa) in length. There are two NAD binding sites (Asp106 and Asn388) with a catalytic region between aa 223-226. One splice variant shows a nine aa insertion after Ser220. Over aa 75-222, human GAPDH-2 shares 84% aa identity with mouse GAPDH-2.
Long Name
Glyceraldehyde-3-phosphate Dehydrogenase 2
Alternate Names
GAPD2, GAPDH2, GAPDHS, HSD-35
Gene Symbol
GAPDHS
UniProt
Additional GAPDH-2 Products
Product Specific Notices for Human GAPDH-2 Alexa Fluor® 647-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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