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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 671708

Product Specifications

Immunogen

E. coli-derived recombinant human GADD153
Met1-Ala169
Accession # P35638

Specificity

Detects human GADD153 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human GADD153 Alexa Fluor® 350-conjugated Antibody

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: GADD153

GADD153, also known as CCAAT-enhancer binding protein zeta (C/EBPz), DDIT, and CHOP, is a ubiquitous 29 kDa basic-leucine zipper transcription factor. GADD153 heterodimerization with other C/EBP factors inhibits their activity and alters their DNA sequence recognition. GADD153 inhibits Wnt signaling by dimerizing with the transcription factors TCF-3 and TCF-4. It is upregulated under conditions of cellular stress including DNA damage, oxidative damage, nutrient depletion, ER stress, and mechanical stress, whereupon it promotes the apoptotic response. The transcriptional activity of GADD153 is enhanced by phosphorylation at Ser 78 and Ser 81. Human GADD153 shares 88% and 90% amino acid sequence identity with mouse and rat GADD153, respectively.

Long Name

Growth Arrest and DNA Damage-inducible Protein GADD153

Alternate Names

CEBP zeta, CHOP, CHOP10, DDIT3

Entrez Gene IDs

1649 (Human); 13198 (Mouse); 29467 (Rat)

Gene Symbol

DDIT3

UniProt

Additional GADD153 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human GADD153 Alexa Fluor® 350-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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