Recombinant Human Flt-3 Ligand/FLT3L (Catalog # 308-FK) stimulates proliferation in BaF3 mouse pro-B cell line transfected with mouse Flt-3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Flt-3 Ligand/FLT3L (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Flt-3 Ligand/FLT3L Monoclonal Antibody (Catalog # MAB608). The ND₅₀ is typically 0.02-0.06 µg/mL.

Human Flt-3 Ligand ELISA Standard Curve

Recombinant Human Flt-3 Ligand (Catalog # 308-FKN) was serially diluted and captured by Mouse Anti-Human Flt-3 Ligand/FLT3L Monoclonal Antibody (Catalog # MAB608) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Flt-3 Ligand/FLT3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-308-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human Flt-3 Ligand/FLT3L Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Flt‑3 Ligand/FLT3L (Catalog # 308-FK)

Neutralization

Measured by its ability to neutralize Flt‑3 Ligand/FLT3L-induced proliferation in BaF3 mouse pro‑B cell line transfected with mouse Flt‑3. Hannum, C. et al. (1994) Nature 368:643. The Neutralization Dose (ND₅₀) is typically 0.02-0.06 µg/mL in the presence of 1 ng/mL Recombinant Human Flt‑3 Ligand/FLT3L.

Human Flt-3 Ligand/FLT3L Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Human Flt-3 Ligand/FLT3L Biotinylated Antibody (Catalog # BAF308)
Standard: Recombinant Human Flt-3 Ligand/FLT3L Protein (Catalog # 308-FKN)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Flt-3 Ligand/FLT3L

Flt-3 Ligand, also known as Flk-2 Ligand, is a transmembrane protein that can be cleaved to release a soluble cytokine. Flt-3 Ligand binds Flt-3 and promotes the growth and differentiation of early hematopoietic progenitor cells.

Long Name

fms-like Tyrosine Kinase 3 Ligand

Alternate Names

FLG3L, Flt3L, FLT3LG

Entrez Gene IDs

2323 (Human); 14256 (Mouse); 102125470 (Cynomolgus Monkey); 493796 (Feline)

Gene Symbol

FLT3LG

UniProt

AAA17999

Additional Flt-3 Ligand/FLT3L Products

Product Documents for Human Flt-3 Ligand/FLT3L Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Flt-3 Ligand/FLT3L Antibody

For research use only

Citations
Reviews

Human Flt-3 Ligand/FLT3L Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB608

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Endotoxin Level

Scientific Data Images for Human Flt-3 Ligand/FLT3L Antibody

Cell Proliferation Induced by Flt-3 Ligand/FLT3L and Neutralization by Human Flt-3 Ligand/FLT3L Antibody.