Human FGFR2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6842
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 98742
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human FGF R2 isoforms and Mouse myeloma cell line NS0-derived recombinant human FGF R2 isoforms
Specificity
Detects all isoforms of human FGF R2 in direct ELISAs and Western blots. Does not cross‑react with any isoform of mouse FGF R2 or human FGF R1, FGF R3, or FGF R4.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human FGFR2 Antibody
Detection of FGFR2 by Western Blot
Inhibitory effects of regorafenib, M‐2, and M‐5 in cell‐based mechanistic assays. (A) VEGFR2 autophosphorylation in HuVECs. (B) VEGFR3 autophosphorylation in HuLECs and effects on potential intracellular signaling kinases ERK1/2 and AKT. *Indicates unspecific signals. (C) Inhibition of cell migration, analyzed by scratch assay in HuLECs. Black lines demarcate the borders of the confluent cell layer. (D) Inhibition of FGFR2 autophosphorylation in SNU‐16 tumor cells. Regorafenib data in A, B, and C were taken from Schmieder et al. 14. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27734608), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human FGFR2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human FGF R2 alpha (IIIb) Fc Chimera (Catalog # 663-FR)
Recombinant Human FGF R2 alpha (IIIc) Fc Chimera (Catalog # 712-FR)
Recombinant Human FGF R2 beta (IIIb) Fc Chimera (Catalog # 665-FR)
Recombinant Human FGF R2 beta (IIIc) Fc Chimera (Catalog # 684-FR)
Sample: Recombinant Human FGF R2 alpha (IIIb) Fc Chimera (Catalog # 663-FR)
Recombinant Human FGF R2 alpha (IIIc) Fc Chimera (Catalog # 712-FR)
Recombinant Human FGF R2 beta (IIIb) Fc Chimera (Catalog # 665-FR)
Recombinant Human FGF R2 beta (IIIc) Fc Chimera (Catalog # 684-FR)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGFR2
FGF R2 is one of four closely related transmembrane tyrosine kinases (FGF R1-4) that fuction as receptors for the fibroblast growth factor family. Multiple isoforms are generated by alternative mRNA splicing resulting in extracellular domains with three ( alpha isoforms) or two ( beta isoforms) Ig-like domains. In addition, alternative exon usage in the Ig III (membrane proximal) domain results in IIIb or IIIc isoforms.
Long Name
Fibroblast Growth Factor Receptor 2
Alternate Names
CD332, FGF R2
Gene Symbol
FGFR2
Additional FGFR2 Products
Product Documents for Human FGFR2 Antibody
Product Specific Notices for Human FGFR2 Antibody
For research use only
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