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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 98739

Product Summary for Human FGFR2 Antibody

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human FGF R2 isoforms and mouse myeloma cell line NS0-derived recombinant human FGF R2 isoforms

Specificity

Detects all isoforms of human FGF R2 ( alpha and beta, IIIb and IIIc). In direct ELISAs, approximately 25‑50% cross-reactivity with mouse FGF R2 and no cross-reactivity with any isoform of recombinant human (rh) FGF R1, rhFGF R3, or rhFGF R4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human FGFR2 Antibody

FGF R2 antibody in Human Adenocarcinoma by Immunohistochemistry (IHC-P).

FGF R2 in Human Adenocarcinoma.

FGF R2 was detected in immersion fixed paraffin-embedded sections of human adenocarcinoma using Mouse Anti-Human FGF R2 Monoclonal Antibody (Catalog # MAB6843) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
FGF R2 beta Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R2 Antibody.

FGF R2 beta Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R2 Antibody.

Recombinant Human FGF R2 beta (IIIc) Fc Chimera (Catalog # 684-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R2 beta (IIIc) Fc Chimera (6 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R2 Monoclonal Antibody (Catalog # MAB6843). The ND50 is typically 0.1-0.5 µg/mL in the presence of heparin (10 µg/mL).

Applications for Human FGFR2 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Kato III human cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver cancer tissue and human adenocarcinoma

Neutralization

Measured by its ability to neutralize FGF R2 beta-mediated inhibition of proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 6 ng/mL Recombinant Human FGF R2 beta (IIIc) Fc Chimera, 0.3 ng/mL Recombinant Human FGF acidic, and 10 µg/mL heparin.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 2 publications using MAB6843 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGFR2

Fibroblast Growth Factor Receptor 2 (FGF R2) is one of four closely related transmembrane tyrosine kinases (FGF R1‑4) that function as receptors for the fibroblast growth factor family. Multiple isoforms are generated by alternative mRNA splicing resulting in extracellular domains with three ( alpha isoforms) or two ( beta isoforms) Ig-like domains. In addition, alternative exon usage in the Ig III (membrane proximal) domain results in IIIb or IIIc isoforms.

Long Name

Fibroblast Growth Factor Receptor 2

Alternate Names

CD332, FGF R2

Entrez Gene IDs

2263 (Human); 14183 (Mouse)

Gene Symbol

FGFR2

Additional FGFR2 Products

Product Documents for Human FGFR2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human FGFR2 Antibody

For research use only

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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