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Human FGF‑BP Antibody
Catalog # MAB1593 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 196519
Product Summary for Human FGF‑BP Antibody
Immunogen
E. coli-derived recombinant human FGF-BP
Val35-Cys234
Accession # Q14512
Val35-Cys234
Accession # Q14512
Specificity
Detects human FGF-BP in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant rat FGF-BP is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human FGF‑BP Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human FGF-BP (Catalog # 1593-FB)
Sample: Recombinant Human FGF-BP (Catalog # 1593-FB)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Published Applications
Read 6 publications using MAB1593 in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF-BP
Fibroblast growth factor binding protein (FGF‑BP), also known as HBp17, is a secreted glycoprotein that increases the bioavailability of FGFs (1). Mature FGF-BP is a 34 kDa, 211 amino acid (aa) O-glycosylated protein with five conserved intrachain disulfide bonds (2‑4). FGF-BP contains a heparin-binding domain (aa 110‑143) and a distinct FGF-binding region (aa 193‑243) (5). Mature human FGF‑BP shares 59% and 54% aa sequence identity with mouse and rat FGF-BP, respectively. FGF-BP is expressed throughout development and in adult squamous epithelium (2, 6). It is upregulated in injured skin, renal tubular epithelium, and spinal nerves as well as in carcinomas of the skin, colon, and pancreas (3, 7‑10). FGF-BP binds FGF-1, -2, -7, -10, and -22 which are secreted and sequestered in the extracellular matrix (ECM) (7, 11). The association of FGF-BP with heparan sulfate proteoglycans (HSPG) weakens HSPG attachment of FGFs and promotes their release (2, 8, 12, 13). FGF‑BP enhances the mitogenic effects of FGFs, thereby contributing to epithelial, endothelial, and neuronal tissue repair, angiogenesis, and tumor growth (7‑9, 11, 14, 15).
References
- Abuharbeid, S. et al. (2006) Int. J. Biochem. Cell Biol. 38:1463.
- Wu, D. et al. (1991) J. Biol. Chem. 266:16778.
- Tassi, E. et al. (2006) Cancer Res. 66:1191.
- Lametsch, R. et al. (2000) J. Biol. Chem. 275:19469.
- Xie, B. et al. (2006) J. Biol. Chem. 281:1137.
- Aigner, A. et al. (2002) Histochem. Cell Biol. 117:1.
- Beer, H-D. et al. (2005) Oncogene 24:5269.
- Ray, P.E. et al. (2006) Am. J. Physiol. Regul. Integr. Comp. Physiol. 290:R105.
- Tassi, E. et al. (2007) Am. J. Physiol. Regul. Integr. Comp. Physiol. 293:R775.
- Kurtz, A. et al. (2004) Neoplasia 6:595.
- Tassi, E. et al. (2001) J. Biol. Chem. 276:40247.
- Mongiat, M. et al. (2001) J. Biol. Chem. 276:10263.
- Kurtz, A. et al. (1997) Oncogene 14:2671.
- Aigner, A. et al. (2001) Int. J. Cancer 92:510.
- Czubayko, F. et al. (1997) Nat. Med. 3:1137.
Long Name
Fibroblast Growth Factor Binding Protein
Alternate Names
FGF-BP1, FGFBP, FGFBP-1, FGFBP1, HBp17
Gene Symbol
FGFBP1
UniProt
Product Documents for Human FGF‑BP Antibody
Product Specific Notices for Human FGF‑BP Antibody
For research use only
Citations for Human FGF‑BP Antibody (6)
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Citations are publications that use Bio-Techne products. Selected citations for Human FGF‑BP Antibody include:
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Species: Human
Sample Types: Cell Lysates
Applications: Western BlotY Kong et al. (2022-02-28), Histone Deacetylase Inhibitors (HDACi) Promote KLF5 Ubiquitination and Degradation in Basal-like Breast Cancer International journal of biological sciences, 2022-018(5):2104-2115.
PMID: 35342356 -
Species: Human
Sample Types: Serum
Applications: ELISA CaptureX Chen et al. (2021-08-02), Poly-L-arginine promotes asthma angiogenesis through induction of FGFBP1 in airway epithelial cells via activation of the mTORC1-STAT3 pathway Cell Death & Disease, 2021-012(8):761.
PMID: 34341336 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotY Wu et al. (2019-10-17), USP3 promotes breast cancer cell proliferation by deubiquitinating KLF5 J. Biol. Chem., 2019-10(0).
PMID: 31624151 -
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western BlotW Zhou et al. (2017-04-24), miR-217 inhibits triple-negative breast cancer cell growth, migration, and invasion through targeting KLF5 PLoS ONE, 2017-012(4):e0176395.
PMID: 28437471 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotZhi X et al. (2012-06-01), YAP promotes breast cell proliferation and survival partially through stabilizing the KLF5 transcription factor. Am. J. Pathol., 2012-0180(6):2452-61.
PMID: 22632819 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotJaeger J et al. (2007-02-01), Gene expression signatures for tumor progression, tumor subtype, and tumor thickness in laser-microdissected melanoma tissues. Clin. Cancer Res., 2007-013(3):806-15.
PMID: 17289871
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