FFAR1/GPR40 was detected in immersion fixed paraffin-embedded sections of human brain hypothalamus using Mouse Anti-Human FFAR1/GPR40 Monoclonal Antibody (Catalog # MAB11025) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human FFAR1/GPR40 Antibody

Application

Recommended Usage

Immunohistochemistry

3-25 µg/mL
Sample: paraffin-embedded sections of human hypothalamus

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FFAR1/GPR40

Human Free Fatty Acid Receptor 1 (FFAR-1), also known as GPR40, is a class A G-protein coupled receptor (GPCR) for medium and long chain saturated and unsaturated fatty acids encoded by the FFAR1 gene. FFAR-1 is expressed in pancreas and it plays an important role in glucose homeostasis acting as a nutrient sensor. Fatty acid binding increases glucose-stimulated insulin secretion, and may also enhance the secretion of glucagon-like peptide 1 (GLP-1).

References

Long Name

Free Fatty Acid Receptor 1

Alternate Names

FFA1, GPR40

Entrez Gene IDs

2864 (Human); 233081 (Mouse); 266607 (Rat)

Gene Symbol

FFAR1

UniProt

O14842 (Human)

Additional FFAR1/GPR40 Products

Product Documents for Human FFAR1/GPR40 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human FFAR1/GPR40 Antibody

For research use only

Citations
Reviews

Human FFAR1/GPR40 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11025

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all FFAR1/GPR40 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human FFAR1/GPR40 Antibody

Detection of FFAR1/GPR40 in Human Brain Hypothalamus.