Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)

Antibody Source

Polyclonal Sheep IgG

View all FCRLA Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FCRLA
Pro270-Glu359
Accession # Q7L513

Specificity

Detects human FCRLA in direct ELISAs and Western blots. In direct ELISAs, approximately 5-10% cross-reactivity with recombinant mouse FCRLA is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human FCRLA Alexa Fluor® 594-conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: FCRLA

FCRLA (Fc Receptor-Like A; also known as FcRX, FcRL1 and Fc receptor Homolog Expressed in B cells/FREB) is a 40-44 kDa intracellular member of the Fc gammaRI class, FcR family, Immunoglobulin Superfamily of molecules. Initially, it is associated with germinal center B cells that are undergoing somatic hypermutation and class-switch recombination. Now, it is believed that all B cells (but not CD38+ plasma cells) are also FCRLA+. FCRLA is suggested to act as an ER chaperone during antibody maturation, and is known to bind to IgM, IgA, and IgG prior to their secretion. Human FCRLA is synthesized as a 359 amino acid (aa) precursor that contains a 27 aa signal sequence plus a mature region that contains two C2-type Ig-like domains (aa 70-159 and 170-257), and a C-terminal poly-Proline region (aa 269-315). Although there is no traditional ER retention signal, a viable substitute is assumed to exist in the N-terminus of the mature molecule. FCLRA is believed to exist naturally as a monomer; however, disulfide-linkage can occur during experimental manipulation. At least eight potential isoform variants have been reported. It is unclear if, or how frequently they are expressed. One utilizes an alternative start site 17 aa upstream of the standard site, a second contains a six aa insertion after Ala28, and a third possessess an four aa substitution for aa 29-166. Five show single block deletions; in random order, they involve aa 78-166, 27-166, 78-261, 21‑261 and 167-26. Over aa 270-359, human FCLRA shares 55% aa sequence identity with mouse FCRLA.

Long Name

Fc Receptor-like A

Alternate Names

FCRLc1, FCRLM1, FCRLX, FcRX, FREB

Entrez Gene IDs

84824 (Human); 98752 (Mouse)

Gene Symbol

FCRLA

UniProt

Q7L513

Additional FCRLA Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human FCRLA Alexa Fluor® 594-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.